We narrowed to 207 results for: Kars;
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TypeBlog Post..., Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell...
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Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...State University, Indiana University, and the Karolinska Institute developed mNeonGreen via 21 substitutions... -
Plasmids 101: Origin of Replication
TypeBlog Post...simplicity’s sake, we've ignored those used in eukaryotic cells and viruses and focused only on those found... -
Another Pathway into Cells: iTOP
TypeBlog Post...Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many complex cellular... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post... students are not even allowed to manipulate prokaryotic cells? Especially, if you only have 4 hours and... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...sequence and ultimately its degradation. While other eukaryotes don’t naturally have this response, they do contain... -
Plasmids 101: Mammalian Vectors
TypeBlog Post... 100-400 ug/mL *Not comprehensive. ** In eukaryotes. ***The concentration used for selection is... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...https://doi.org/10.1093/ilar.46.3.258 Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ ... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P., Zhang, F. (2015). Cpf1... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk is a... -
Antibodies 101: Affinity Reagents
TypeBlog Post...monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used as intracellular... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...Autophagy. Methods in Enzymology Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, 33-42. doi... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic...9 system may be re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post... Karen Guerin... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA passengers.... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...hear it (whether at a scientific conference or a karaoke bar), you probably want to sigh. The problem is... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ...