We narrowed to 206 results for: Kars;

1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   ...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

2. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   ...previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a variety of natural...

3. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   ..., Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell...

4. Meet the New Addgene Board Members!

   Type

   Blog Post

   ... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge...

5. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   ...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...

6. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   ...State University, Indiana University, and the Karolinska Institute developed mNeonGreen via 21 substitutions...

7. Plasmids 101: Origin of Replication

   Type

   Blog Post

   ...simplicity’s sake, we've ignored those used in eukaryotic cells and viruses and focused only on those found...

8. Another Pathway into Cells: iTOP

   Type

   Blog Post

   ...Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H...

9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

   Type

   Blog Post

   ...of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many complex cellular...

10. "Build Your Plasmid": The Game - Play to Teach

    Type

    Blog Post

    ... students are not even allowed to manipulate prokaryotic cells? Especially, if you only have 4 hours and...

11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    ...sequence and ultimately its degradation. While other eukaryotes don’t naturally have this response, they do contain...

12. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    ... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

13. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    ...https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ ...

14. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ... Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1...

15. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    ... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided...

16. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    ...Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk is a...

17. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used as intracellular...

18. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    ...Autophagy. Methods in Enzymology Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, 33-42. doi...

19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    ...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ...

20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type

    Collection

    ... tagged recombinant protein pDest-Sumo - For prokaryotic expression of target protein as N-terminal fusion...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...