Plasmids 101: Multicistronic Vectors
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Blog Post
...downstream.4 The "cleavage" occurs between the Glycine and Proline residues found on the C-terminus meaning...
We narrowed to 146 results for: LYC
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Tips for arabidopsis transformation
TypeBlog Post...starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts and ... -
Plasmids 101: Colony PCR
TypeBlog Post...standard for reference and to add a loading dye with glycerol to your samples before pipetting them onto the... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...generation of the transfer vector. Q5: What envelope glycoprotein will be expressed on virus particles? A5: This... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post... Selecting the Right Antibody Antibodies 101: Polyclonal Antibodies Resources on addgene.org The Addgene... -
Viral Production at Addgene
TypeBlog Post...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin screen... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic amino ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... usually 15-20 amino acids long and made up of glycine and serine with dispersed hydrophilic residues ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...post-translational modifications on those proteins, like glycosylation patterns. Surface labeling is particularly useful... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...are directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this approach... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...ataxia telangiectasia, congenital disorder of glycosylation, and Niemann-Pick disease type C. Although the... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG If you need more... -
Quick Guide to All Things Lentivirus
TypeBlog Post...protocols you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol...