We narrowed to 149 results for: LYC

1. Viral Production at Addgene

   Type

   Blog Post

   ...preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated ...

2. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...post-translational modifications on those proteins, like glycosylation patterns. Surface labeling is particularly useful...

3. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   ...are directed to integrins that bind the Arginine-Glycine-Aspartate (RGD) amino acid sequence in their ligands...

4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   ...unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic amino ...

5. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   ... et al. 2022). Lactate is a key metabolite in glycolysis, so this sensor will be a valuable tool to study...

6. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   ... usually 15-20 amino acids long and made up of glycine and serine with dispersed hydrophilic residues ...

8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   ...transiently using a standard detergent, Polyethylene Glycol (PEG), although the application of this approach...

9. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   ...ataxia telangiectasia, congenital disorder of glycosylation, and Niemann-Pick disease type C. Although the...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ...acids1 AAV receptor2   AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (not strictly required)...

11. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    ... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more...

12. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    ...protocols you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol...

13. Institute for Protein Innovation

    Type

    Collection

    ... Glypicans are a family of highly conserved proteoglycans crucial for regulating cell signaling during...

14. SARS-CoV-2 Pseudotyped Virus

    Type

    Collection

    ...virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped virus particles...

15. CRISPR Plasmids - Base Edit

    Type

    Collection

    ...that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived...

16. AAV for Neuronal Tracing

    Type

    Collection

    ...For example, G-deleted rabies lacks the viral glycoprotein (G) gene, which is not required for the transcription...

17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    ...various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently attached to...

18. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    ...conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify...

19. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ...liver and ameliorates streptozotocin-induced hyperglycemia. Nat Med, 2000. 6(5): p. 568-72. PubMed PMID...

20. Allen Institute for Cell Science Plasmid Collection

    Type

    Collection

    ...nucleoids 164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP...