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We narrowed to 12 results for: LYC

Showing: 1 - 12 of 12 results

  1. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term...time, you will need to establish glycerol stocks. The addition of glycerol stabilizes the frozen bacteria...of 50% glycerol in a 2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution...concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can prepare...your glycerol stock before you begin your plasmid mini-prep. Try not to freeze/thaw your glycerol stock...Protocol for creating a glycerol stock, including additional tips for long-term storage. Protocols...Bacterial Culture Background Information Bacterial glycerol stocks are important for long-term storage of ...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...how to generate a monoclonal cell line from a polyclonal pool of stable cells....how to generate a monoclonal cell line from a polyclonal pool of stable cells. Transducing cells with ...with lentivirus results in a heterogenous polyclonal population that varies in the number of integration ... as the lower expressing clones take over the polyclonal cell pool. Generating a monoclonal cell line ... Hemocytometer or other cell counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10...FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol for generating...cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is always undergoing selection, it’...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...cells, resulting in a more homogenous (but still polyclonal) cell population. Depending on the transducibility...regularly to confirm transgene expression. As polyclonal populations of resistant cells start proliferating...etc. Pro-Tip This selection method results in a polyclonal cell population, meaning that the transgene has...from different cells in the population. Once the polyclonal populations are growing well and have been sufficiently...expression. Over time, transgene expression in a polyclonal population may drop. This is because cells that...consider generating monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation...

  4. Protocol - How to Streak a Plate

    Type
    Protocol
    ...from Bacterial Culture Introduction If you have a glycerol stock or stab culture of bacteria and need to ...burner. Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile loop, pipette tip or toothpick...punctured area of the stab culture or the top of the glycerol stock. Pro-Tip If you use a wire loop you can ...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...Lithium Chloride (for precipitation step, Option B) Glycogen (Optional) Caution Make sure to read the SDS (...anticipate your RNA yield to be small, RNase-free Glycogen may be used as a carrier to facilitate RNA precipitation...

  6. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering... the bacteria, you can proceed with Creating a Glycerol Stock . You can now isolate your plasmid DNA from...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM... and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the individual Maxipreps...
Showing: 1 - 12 of 12 results