We narrowed to 163 results for: NAP

1. Hot Plasmids - February 2022

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   ...anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies...

2. Viral Vectors 101: The AAV Retrograde Serotype

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   ... neurons and six hundred trillion connections (synapses.)  In this post we’ll describe the AAV retrograde...

3. Sharing is Caring: But How to Distribute Open Hardware?

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   ...optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for...

4. Fluorescent Proteins 101: Luciferases

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   ... (luciferin),730–743 (analogs) 64 Luciferin or naphthyl-luciferin analogs ATP No Deep sea copepod (Gaussia...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase TadA-7.10 A mutagenesis...

6. A Deep Dive into BioBuilder

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   Blog Post

   ...and in more than 30 countries around the world. Snapshots of the BioBuilder experience With its intentionally...

7. Plasmids 101: Methylation and Restriction Enzymes

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   ...illustrates how sequence visualization software (we used Snapgene here) can help you assess whether your restriction...

8. Seven Tips for Using LinkedIn as a Scientist

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   Blog Post

   ...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

9. RANbodies: Reporter Nanobody Fusions

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   Blog Post

   ... GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No antibody required...

10. RNA Extraction Without A Kit

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    Blog Post

    ...These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions...

11. Addgene's Tips for Plasmid Quality Control

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    ...examples include Benchling, Serial Cloner, and Snapgene), and save these to a shared computer or server...

12. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...and Nancy Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor...

13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ...for the visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared...

14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...selectable markers by detecting common features using Snapgene. If we find information that differs from what...

15. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...spider silk, or the activity of a protein in a synapse. What kinds of tools enable us to measure these...

16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...neurons, and are especially abundant in neuronal synapses. shRNA silencing of one circular RNA they have...

17. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...et al., 2018). Think of 3C methods as taking a snapshot of chromatin looping with the photo “developed...

18. Transferable Skills: Negotiation

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    ...Bruce Patton More books on negotiation  Skills Snapshot: Negotiations When Cultures Collide: Managing ...

19. Degrading DNA with Cascade-Cas3

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    ...Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade...

20. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) ...