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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 238 results for: SPL

Showing: 101 - 120 of 238 results

  1. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...versions with both PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments...biocytin-filled cell expressing BiPVe3-dTomato, displaying characteristic PV basket cell morphology. Adapted...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

  4. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...

  8. Retrovirus Plasmids

    Type
    Collection
    ...to produce infectious viral particles have been split into multiple plasmids for safety: Transfer plasmid...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... reverse transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...emission maximum. Note, you can also use the tool to display excitation spectra, which I excluded here for better...created through the actual APC-Cy7 fluorophore but displays an emission spectrum that overlaps with APC-Cy7...

  11. Worm Expression Resources

    Type
    Collection
    ...ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg Lab. Plasmids for...

  12. Promega Plasmid Collection

    Type
    Collection
    ...of test compounds is measured by competitive displacement of a NanoBRET tracer, reversibly bound to a ...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...because the top figure shows the construct from our 'Split RNA' design, where the pre-processing form of crRNA... more details. One important note is that this ‘split RNA’ design is not as efficient in genome editing...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...membrane and gel during the transfer, while dark splotches could indicate dirty equipment, degraded or improperly...lysis Add sonication step to protein lysis Splice variants Check literature Posttranslational...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Rajat Rohatgi 107855 pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G) TARDBP CMV ALS Rajat...Rajat Rohatgi 107860 pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) TARDBP CMV ALS Rajat... VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm...Bernhard Lechtenberg 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd...

  16. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...station if unwanted or biohazardous materials are splashed onto your face and/or get into your eyes. Wash...shaking or mixing, which may produce aerosols or splashes. These procedures should be done underneath a ...

  17. Caltech Systemic Capsids

    Type
    Collection
    ...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...improved peroxidase reporter, APEX2, through yeast display. APEX2 has been shown to exhibit superior performance...functional complementation between two non-fluorescent split GFP fragments. When the two fragments, expressed...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ... of PEG solution to each 100 mL of supernatant. Split into 2 x 500 mL sterile bottles as needed. Add stir...cells were imaged with a 10X objective and were split 2 days before these images were taken. Last reviewed...
Showing: 101 - 120 of 238 results