We narrowed to 402 results for: SUA

... luck! You don’t need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion...deletion large enough that it can be visualized by running your PCR on a gel. It’s still paramount that...amplicon isn’t too large so that you can easily visualize the size shift you anticipate. A good rule of ...

...trained to have these types of negotiations. They usually turn into “win-lose” negotiations. This leads to... together. The problem is that the discussions usually focus on the numbers, rather than the necessary...weight. Therefore, too much focus on money can dissuade a productive negotiation. A more productive discussion...

...ways to ultimately achieve success, we find it is usually faster and easier to do the validation first.  ... efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes...titer” is required for efficient AAV transduction, usually without any numbers for context. That’s because...

...by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following...however, is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent.... This means that, for gene editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference...

... history, fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening...these fluorophores to enable highly specific visualization of cellular components, typically a protein ...Alexa Fluor 488, and thus it is more difficult to visualize the stain from the background. Dyes that can pass...

...that many people honor. Talking with strangers is usually challenging but does get easier with practice. ... by meeting with people working in diverse and unusual fields. My favorite final trick is to end each ...

...difficult to study without biosensors to directly visualize their activity. In 2019, the first generation ...developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019). But this first...

... the paper. Delivery of AAV into the brain can usually be achieved by a directed brain injection, but ...strategies can also be used for retrograde tracing: visualizing neurons that received information from a region...

...labeling; multicolor labeling would enable the visualization of multiple loci. Such a system could then be...major applications to cancer, for example, the visualization of chromosomal translocations or chromosomal...

...to apply for a position.  A job description is usually a wish list and the hiring manager knows that no... know the exact person monitoring submissions (usually a Human Resources representative). Pro tip: if ...

...use an RNA guide to target other nucleic acids—usually DNA—to cut, nick, or bind. DNA CRISPR array: The...previously encountered foreign nucleic acids. The unusual sequence of these arrays, with alternating spacers...

...genetically fused to fluorescent proteins and used to visualize target antigens in living cells or tissues. sdAbs...the process started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable...

...labeling fewer cells in a population, it’s easier to visualize individual/non-overlapping cells. While transgenic...Drosophila, the SPARC systems can be used both for visualization and for optogenetics. The Clandinin lab used...

...techniques and cool plasmids. Creating graphics were usually the last thing I thought about when writing posts...file size with a Biorender watermark, which is usually ok for informal settings like lab meeting presentations...

... file into the desktop version of Snapgene to visualize the sequence and look for features. The first ...extensive database. Snapgene also provides easy visualization of open reading frames, which is often our first...

...imaging with fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail... lux. arg1 and lux reporters both allow for visualization of the bacteria, but lux imaging shows only ...

... called TRUPATH to interrogate 14 of the 16 non-visual G protein signal transducers (collectively known...optimal Rluc8-Gα construct for two of the 16 non-visual G protein transducers. To use the TRUPATH system...

...delivery to be viral-mediated. Transformations are usually bacterial in nature. All three of these methods...packaging plasmids into a viral production cell line, usually HEK-293 cells. When all of these viral plasmids...

...working with antibodies.) Conformation changes usually fall into one of two categories: ones induced by...or fold in certain conditions? It can help to visualize an abstract protein during your sample prep process...

...PMID 29379139. The “Riboglow” platform for visualizing RNA production and localization Article contributed...Riboglow,” a set of tools that can be used to visualize RNA localization in mammalian cells. These tools...