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We narrowed to 420 results for: SUA

Showing: 101 - 120 of 420 results

  1. Technical Design of a Western Blot

    Type
    Blog Post
    ...than nitrocellulose membranes. Pro tip! You can visualize proteins on a PVDF membrane without staining; ...protein (HRP) and alkaline phosphates. HRPs are usually preferred because of their higher specific activity...chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly different amounts in the same...background Direct detection method Less sensitive; usually more expensive Nitrocellulose or low-fluorescence...

  2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...is a dangerous state for the body to be in and usually self-limiting effects kick in trying to resolve...and Defense With increasingly sophisticated visualization techniques it is possible to follow the interactions.... PubMed PMID: 27913448. 3. Cai, En, et al. "Visualizing dynamic microvillar search and stabilization ...

  3. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...have introduced a GFP protein into your cells (visualized via the FITC channel) and you are trying to see...negative control. You can see how it is easier to visualize these four populations in the histogram plot in...gate off the histograms above, allowing you to visualize your data as a dot plot.  To summarize, if you...

  4. Viral Vectors

    Type
    Collection
    ...genes that are required for viral replication are usually divided among many plasmids, such that all of these...

  5. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...met with several individual and/or small groups, usually starting with the hiring manager. These interviews...setting, a conflict, and a resolution. Stories usually have a theme too, which is something not inherently... 5-6 stories for an interview, since one story usually has more than one theme and can be used to answer...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...sites. This new technology opens up a new way to visualize changes in gene expression and may lead to the...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    ...mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time. Examples ...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    ...April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric population...However, here it appears that the culprit for these unusual cases was either 1) a physiological effect of SARS-CoV...transgene) instead.    Although the late genes are usually present in the transfer plasmid for the majority...

  11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...interests include proteomics, machine learning, and visualization for big biology. GPS for the genome We can think...something like Google Maps to help us identify and visualize addresses. That’s where enhancers and genome math...result is a heterogenous population of stem cells, usually housed in a single flask. Through a bit of stochastic...

  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...steal all their food from other plants and are unusual enough to be interesting biologically. They have...squid has an extreme number of nerve cells and an unusually high level of RNA recoding in the brain. RNA editing...each other in captivity. Sticklebacks demonstrate unusual parallel adaptation to disparate environments so...

  13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...and temporal resolution, making it ideal for visualizing gene expression in vivo.  But what ultrasound...genes that, when expressed, enable cells to be visualized under ultrasound imaging. Expression of ARGs ...first-generation mammalian ARGs (mARGs) enabled ultrasound visualization of in situ mammalian gene expression in deep...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...the time of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There...Unfortunately, the symptoms of mesothelioma are usually very similar to the symptoms of other diseases,... meaning that by the time it has caught it has usually started to spread (metastasize) and turned into...

  15. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...divided into two halves that are distinguished visually through different patterns and the animal only...the opposite chamber) [2]. Laser activation is usually controlled automatically using motion capture software...electric foot shock that has become associated with a visual or auditory cue. The hippocampus has long been ...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... luck! You don’t need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion...deletion large enough that it can be visualized by running your PCR on a gel. It’s still paramount that...amplicon isn’t too large so that you can easily visualize the size shift you anticipate. A good rule of ...

  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...trained to have these types of negotiations. They usually turn into “win-lose” negotiations. This leads to... together. The problem is that the discussions usually focus on the numbers, rather than the necessary...weight. Therefore, too much focus on money can dissuade a productive negotiation. A more productive discussion...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following...however, is a very low-efficiency process, and usually involves the need for single cell cloning and subsequent.... This means that, for gene editing, there are usually few potential gRNAs. While SpCas9, with a PAM preference...

  19. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...ways to ultimately achieve success, we find it is usually faster and easier to do the validation first.  ... efficiently transduce your cells. In fact, we usually recommend testing more than one.  Most AAV serotypes...titer” is required for efficient AAV transduction, usually without any numbers for context. That’s because...
Showing: 101 - 120 of 420 results