...tested GeoCas9’s love of heat in two ways. The first set of experiments tested the ability of GeoCas9 RNPs...maintain activity following heat treatment. The second set of experiments tested the ability of GeoCas9 RNPs...

... the team and bring on scientists with a diverse set of experiences. Let’s meet the newest board members...through direct industry collaborations, grants, or setting up commercial and nonprofit companies. His lab ...

...polymorphisms Codon optimization tool: To access this tool, set up an IDT account. Figure 2: Addgene's Analyze...Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning... of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository for fluorescent...

...remove the plate seal from the 96-well plate and set aside. Aspirate the wells and use a multichannel ...the plate. Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10...a plate seal and incubate on a microplate shaker set to 400 rpm for 2 h at room temperature or overnight...the plate Place the plate on a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 18–...

...ensure accurate data analysis, include a complete set of controls. Ideally, every flow cytometry experiment...flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation.... The fluorescence-minus one panel will help you set up gating during data analysis.  We hope that this...analysis and some useful background information for setting up antibody based flow cytometry experiments.   ...cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent...this technology can be used to isolate a specific subset of cells in a population in a procedure termed ...instruments have specialized components that can divert a subset of cells. During FACS, the sample stream oscillates...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers... experts that complement your (scientific) skill set and is therefore very useful for complex projects... point of talking to them at a seminars, or even set up regular meetings. Meeting people outside your ...team or lab, you’re likely to be seen as a huge asset! As is true for any skill, there is a learning curve... from others and meeting people with different mindsets and approaches can truly broaden your mind. When...

... to secure your first (R-level) research grant.  Set your timeline from there, then start identifying ... use these tools Outline the knowledge and skill sets needed to do your science. This will help you identify...entering a win-lose situation that can ultimately set you up for failure. Don’t be afraid to walk away ...with others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...

...Colorado Boulder recently developed “Riboglow,” a set of tools that can be used to visualize RNA localization...colony screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018...

... don’t get a chance apply it in an interactive setting. The problem is…how can you make a “hands-on” ...clinical disease case. Then, each team received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication...

... or create custom protein collections (such as a set of FPs that you or your lab use frequently) for the...the information in the database relating to that set of proteins.  For computer-savvy users looking to...

...fluorescence-based methods require a standard curve, a set of samples with a known DNA quantity and their corresponding... DNA prep. Though this method takes more time to set up in the lab, you might not have to do the calculation...

.... Purpose Gram staining can be used in medical settings to determine the general type of bacteria present...classification system is also useful in a laboratory setting. When designing a plasmid to be inserted into a...

...follow our timelines! Prepare a research plan to set yourself up for success prior to your departure for...reagents are onsite before you need them, and you are set up for success. If you are communicating with a professor...

...Back then, it consisted of “wooden slab benches” set on top of filing cabinets, a rickety old incubator...videos, while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...

...information and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual...

...kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but...

...water bath on and set the appropriate temperature as described in your protocol. How to set the temperature...dial or a digital display you can set to the correct temperature. Set up your water bath so that it will...also helps the water bath heat up quicker. Pro-Tip Set up the water bath 30 minutes to 1 hour before you...

... at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) ... emiRFPs in your applications.  There is a whole set of spectrally distinct (e)miRFPs available. The numbers...Methods 16, 171-174 (2019). Matlashov, M.E. et al. A set of monomeric near-infrared fluorescent proteins for...

...communication to another person.  In an interview setting, this means persuading an employer that your past... to ask and answer these questions for a diverse set of audiences.  Every scientist needs to be able to...skills in science communication will be a positive asset throughout your career? 1. Make scicomm practice...of this as a jumpstart or a refresher for your skillset rather than a permanent fix. Treat every interaction...

... all of biology) require large data sets. Whether these data sets include videos of behavior, many many...