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Showing: 101 - 120 of 618 results

  2. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...polymorphisms Codon optimization tool: To access this tool, set up an IDT account. Figure 2: Addgene's Analyze...Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning... of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository for fluorescent...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    ... the team and bring on scientists with a diverse set of experiences. Let’s meet the newest board members...through direct industry collaborations, grants, or setting up commercial and nonprofit companies. His lab ...

  4. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...tested GeoCas9’s love of heat in two ways. The first set of experiments tested the ability of GeoCas9 RNPs...maintain activity following heat treatment. The second set of experiments tested the ability of GeoCas9 RNPs...

  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...ensure accurate data analysis, include a complete set of controls. Ideally, every flow cytometry experiment...flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation.... The fluorescence-minus one panel will help you set up gating during data analysis.  We hope that this...analysis and some useful background information for setting up antibody based flow cytometry experiments.   ...cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent...this technology can be used to isolate a specific subset of cells in a population in a procedure termed ...instruments have specialized components that can divert a subset of cells. During FACS, the sample stream oscillates...

  6. Bacterial Expression Systems

    Type
    Collection
    ...transcriptional activation or repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial... and Venus) Escherichia coli Wolf Frommer 20336 pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) ...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...Targeted gene activation using SAM There are two sets of 3 vectors each available for mammalian endogenous...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...

  9. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers... experts that complement your (scientific) skill set and is therefore very useful for complex projects... point of talking to them at a seminars, or even set up regular meetings. Meeting people outside your ...team or lab, you’re likely to be seen as a huge asset! As is true for any skill, there is a learning curve... from others and meeting people with different mindsets and approaches can truly broaden your mind. When...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... to secure your first (R-level) research grant.  Set your timeline from there, then start identifying ... use these tools Outline the knowledge and skill sets needed to do your science. This will help you identify...entering a win-lose situation that can ultimately set you up for failure. Don’t be afraid to walk away ...with others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Back then, it consisted of “wooden slab benches” set on top of filing cabinets, a rickety old incubator...videos, while some are looking to develop new skill sets. Kellen Andrilenas is a Ph.D. grad student at Boston...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...

  13. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...follow our timelines! Prepare a research plan to set yourself up for success prior to your departure for...reagents are onsite before you need them, and you are set up for success. If you are communicating with a professor...

  15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...Colorado Boulder recently developed “Riboglow,” a set of tools that can be used to visualize RNA localization...colony screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018...

  16. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... don’t get a chance apply it in an interactive setting. The problem is…how can you make a “hands-on” ...clinical disease case. Then, each team received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication...

  17. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    .... Purpose Gram staining can be used in medical settings to determine the general type of bacteria present...classification system is also useful in a laboratory setting. When designing a plasmid to be inserted into a...

  18. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...information and resources needed for the assignment? Setting someone up for failure by delegating is a bad idea...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual...

  19. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive entries, but...

  20. Water Bath Protocol

    Type
    Protocol
    ...water bath on and set the appropriate temperature as described in your protocol. How to set the temperature...dial or a digital display you can set to the correct temperature. Set up your water bath so that it will...also helps the water bath heat up quicker. Pro-Tip Set up the water bath 30 minutes to 1 hour before you...
Showing: 101 - 120 of 618 results