...step. As with standard IF, you will need to start with a fixed sample. Depending on the sample type...photobleaching or by stripping out the antibodies, then start over with the next target: add the new primary, ...Abcam's sample protocol is a good place to get started.  One important consideration when deciding whether...not being degraded. Whether you’re just getting started with two or three targets or you’re pushing the...variations you could try. We hope this post helped you start planning for your next multiplex IF experiment! ...

... grad school to a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an...to the UK with foundational research, training, startups, and real applications of SynBio. Chapter 3: Synthetic...Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael Koeris Resources...

..., we discuss some of the many things you can do start your own science communication student group and... and Amy say is the quickest way to get things started? Set up your own Science by the Pint series and... best way to get a Science Communication group started. It's as simple as getting people together at a...email [email protected]    For more info about starting your own SciComm organization and to learn about...

...frustrated and no closer to starting your experiments. Get organized now! Start by gathering that pile of...or projects) to make it easier to know where to start your search. Then identify the most frequently used...ways to share your documents with your colleagues. Start small by identifying the protocols, guides, or references...

... picking your research topic, starting a lab, and more. Before we start, we'd like to extend a special... "Choosing Your Research Project," “Advice for Starting Your Lab,” “Fun in the Lab!" and more.   Acknowledgments...Choosing a Research Project Advice for Scientists Starting a Lab ...

...expression, is it necessary to add a G nucleotide to the start of my target sequence? A2: The human U6 promoter...promoter prefers a 'G' at the transcription start site to have high expression, so adding this G could help ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...that mutation be selected/screened? A4: Before starting your experiment, consider co-transfecting with...

...agreed to come in and start getting plasmid deposits into the collection and to start shipping. They created...closure was earlier than most companies. Since the start of this crisis, we’ve been very responsive to the...Addgenies home.  At the same time, as Addgene started dealing with this new reality of working remotely...

...alternative” anymore.   Since most scientists start out in academia, it is especially important for ...calendar Academic researchers must be extreme self-starters and especially good at self-promoting—No one is...about something like this without sufficient data? Starting to learn about the diverse options and how to ...Science Careers How to Negotiate a Successful Lab Start Up Budget Read Our Management for Scientists Series...

...their position relative to a transcription start site. In STARR-seq, randomly sheared genomic libraries ...(Q3) MARCH 2016Enhancer activity mapping with STARR-seq   Although many technologies have been developed...sequences in different chromatin states. Alexander Stark and colleagues at the Research Institute of Molecular...Pathology have developed an alternative method, called STARR-seq (self-transcribing active regulatory region ...over input levels using next gen sequencing. The STARR-seq backbone is available from Addgene in both human...report using as few as 150 fly neurons as their starting material! Thus, TRIBE can be used to analyze a...

...government sectors.  Within these sectors, you could start your own company, work in a bench research career...sponsorship. If you are in this position, you should start planning for your visa and or green card process...consulting firms), sponsor more H-1Bs than small startups and firms with a non-international focus. Candidates...applicants received sponsorship) then he or she can start working in October of the same year. The Industry...are not already reviewing papers that you should start contacting journal editors to request to be a reviewer...

...with the people in your life. At Addgene, we’ve started virtual activities like team lunches, a virtual...your work online. Maybe you’ve always wanted to start using social media professionally but haven’t had...even if your defense isn’t for a while. Getting started early on introductions and literature reviews could...best practices in your research. For a place to start, check out Reproducibility for Everyone. Plan out...

... in the space and decided to reactivate it. We started out by holding a few journal club events and soon...equipment and safety training prior to getting started. For example, Timothy Stiles joined BosLab last...Community projects at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage...Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering Project (OYEP). Through...

...grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of any experiment...experiment. What fixed it was not trying even harder to start earlier, but simply moving all my prep work to the...resources on Addgene’s blog A Guide to Getting Started in Undergrad Research Early Career Researcher Toolbox...

...about Synthetic Biology. Can you tell me where to start?” is a question more and more students are asking... principles to biological systems. Today, what started in Cambridge with a handful of local high school...synthetic biology club of its kind, running from start to finish during the academic year and remaining...Science Department, or to simply tour the lab as a starting point into further STEM exploration.  Further ...

...same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA Perhaps you think...scientific terms when socializing at a conference, but start with a more general intro until you know if the ...problems.  During the conference The conference may start in the airport. If you see someone traveling with...and put your phone away (did I say that already). Start with, “Is this seat taken? Can I join you?” Next...

... drug resistance Although Bhang and co-workers started using the tool to look at a variety of questions...preexisting in the parent population prior to the start of treatment. They hypothesized that, if they labeled... a researcher to label individual members of a starting population of cells with specifc, easily read ... to both drugs. The future of ClonTracer Since starting her new role as an investigator in the In Vivo...

... locus, scientists can mutate the transcription start site, splice sites, exons, and/or promoters to knock... of exons. Disrupting promoter or transcription start site function would also help to reveal the impacts...polyadenylation signal downstream of the transcription start site will terminate transcription of most of the...based approaches to study ncRNAs. (A) Transcription start site (TSS) deletion. (B) Mutations of splice sites...

...robot and to find out how you can get one. As a starting point, I would recommend conferences like Lab ... than you ever knew existed! Addgene recently started automating a few protocols to make standard lab...about 200 vials in 45 minutes by hand. How can you start automating your lab? You might not be doing enough...can do to get into the robotics spirit include: Start using electronic multichannel and single channel...

...antigenic drift.    Starr et al Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science....RBD DMS Library generation and sequencing. From Starr et al Cell., 2020. (B) Mapping antibody-escape mutations... mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV-2 infects host...

... on the hood for at least 15 minutes before you start any tissue culture work and make sure that clean...as well as that watch you’re wearing before you start. Also, avoid unnecessary talking because talking... the hood and can wipe them all down before you start. This will also help you avoid moving things in ...split very soon. There is power in numbers! When starting off with a new cell line, grow the cells, aliquot...