We narrowed to 614 results for: TIL

... cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook from time to...worked weekends and evenings on the project, but still continued to generate new ideas as they popped into... as food but support GMOs that result in a new utility, was there a business opportunity out there for...figure out how we would accomplish the color change until a few months later. RevBio accelerates with help...

...comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and then become...about one week from the initial deposition of the fertilized egg by its mother. Inside the pupal case, the...important to isolate females from males while they are still virgins. Females begin mating ~6 hours after hatching... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. Additional Resources...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently... might be for you. This methodology relies on versatile Type IIS restriction enzymes. These cut outside...mix. Nonetheless, Gibson assembly is a highly versatile and efficient way to generate a wide variety of... is extremely efficient due to the use of the versatile type II restriction enzymes. In Golden Gate cloning...

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

...developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions...instance, the DBD of the fusion protein shown is still able to bind to the upstream activation sequence...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...

...jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and...more about GFP, how scientists have evolved this versatile protein to suit their experimental needs, and ...A. Victoria and expressed in any organism while still maintaining fluorescence. The protein structure,...mainstay in molecular biology. Scientists can easily utilize GFP-containing plasmids as a means to many functional...

... plasmid is delivered to your target cells, you still need to select for the cells that contain it. Antibiotic... approach in a mammalian cell experiment should still include an antibiotic resistance gene for growth...express your protein of interest. However, you do still need a species-compatible promoter for expressing...between prokaryotes and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak...

...2019) If you’ve taken the time to do this and are still struggling with idea generation, try this thought...with an out-of-the-box concept and pare it down until you have something tangible and applicable to your...to the same problem. If your lab/company/team is still working towards a culture of encouraging dissent...easier to work with than nothing at all - they’re still ideas, after all, and that is the goal of this exercise...

..., there still remains the problem of prevention. Asbestos exists pretty much everywhere, still, in old...mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the hope is that...United States and many other countries, and it is still imported, exported, and used throughout the world...

...husbandry and ability to produce abundant, externally fertilized, transparent progeny (Figure 2). These features...regulate processes from development to metabolism to fertility. Several zebrafish-specific gene expression databases...screens produced ~1,500 zebrafish mutants that are still used in labs across the globe (Nüsslein-Volhard ...tracing Over a period of 24 hours, the externally fertilized zebrafish zygote transforms into an organism ...

... and put it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult)....diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming with CRISPR/...

...CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global transcriptional ...rescue the neurological defects of Rett syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett... . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J...398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 ...

...be visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that...homozygous clones, however, sequence confirmation is still needed to verify the correct sequence was inserted...it requires a little extra wet lab work, this is still a great option, especially if the options outlined...data analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing...

...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...in-lab filming and remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth...

... too many moving parts in the whole process to distill a one-size-fits-all recipe for success. What I ...the Dean, and even some people in your field that still need to understand your specific questions and experiments...directly state your official aims, but I suggest distilling them into simple goals/outcomes. This isn't the...show interest in other faculty members’ research. Still a Grad Student? Get Tips on Getting the Most out...

...mutations, we still weren’t entirely happy with our editing outcomes and in many cases still didn’t get ...University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms... be screening a total of a few hundred clones - Still better than 10.000, right? (Kwart, Paquet, et al...

...the Brian Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site ...conducted multiple rounds of directed evolution until they identified a mutant with significantly increased...cells. To circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...

...repair or add in disease-causing mutations, but, until recently it hadn’t been used to treat disease postnatally...the amount of research conducted on DMD, there is still no good treatment. The dystrophin gene is very large...approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option for...CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized...

...used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, piggyBac™ has ... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,...

...specific agonists for orphan GPCRs demonstrating the utility of this platform for drug discovery. Researchers...CCP); the latter of which greatly enhances the utility of this collection as it can be used to easily ... allows for easy assembly of DNA fragments by utilizing regions of sequence homology (usually 30-40bp ... are available at Addgene. Since GMAP assembly utilizes 5 different overlap sites, this technique also... repeats into a single-tube, 3 day process by utilizing pre-assembled trimers to reduce the number of ...appealing tool for studying essential proteins. Until recently, it was challenging to apply the AID-tagging...