...luciferase was published in 1985, several studies utilized luciferase as a genetic reporter in plant and ...None Yes Luminous Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina... produce light, often with a luminometer or scintillation counter, and the activity measurement can then...interest permits translation of functional luciferase, until miRNA target and degrade the mRNA sequence, resulting...

... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using... a reporter and we will be covering this ever-versatile fluorophore in a later post, so stay tuned! Transient...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...selection agent should not be added to culture media until 24-48 h post transfection when creating stable cell...

...focus of the application was changed, but Sample6 still leverages the core engineering and scientific concepts...into the local academic community. However, Mike still could not remove himself from the idea of starting... importantly, “the company does not become real until someone takes the risk full-time and abandons all... many universities throw out old equipment that still works. “We use functional spectrometers from the...

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the.... aureus Cas9 (saCas9) proteins, showing the versatility of this approach. This study generated the first...formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted to regulate gene editing...

...experiments in S phase zygotes at 18 hr post fertilization. They observed higher targeting efficiency than...41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed a repair template ...recessive gene. Ma et al. claim that CRISPR would still be helpful in increasing viable embryos, especially... in terms of editing success rate. Would CRISPR still be worthwhile for a given disease if it increased...

...chemically modified to resist degradation while still effectively serving their function of guiding Cas9...The photoactivatable counterpart to this system utilizes a gRNA which is photocaged and unable to engage...initiate targeted editing events. Both technologies utilize chemical modifications to the RNA to control the...molecule) you will not have that option, but you can still choose what dye to use  What to do once you’ve chosen...

... that the majority of attendees were scientists still working at the bench. It was a fantastic opportunity...unintended themes of the meeting was how much we still don’t know about RNA. One talk that really brought...about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments of these... summed up this conference—nucleic acid science still has many surprises for the researchers in this field...

... cards, incorporation, laboratory lease, etc. I still flip to that page in my lab notebook from time to...worked weekends and evenings on the project, but still continued to generate new ideas as they popped into... as food but support GMOs that result in a new utility, was there a business opportunity out there for...figure out how we would accomplish the color change until a few months later. RevBio accelerates with help...

...comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and then become...about one week from the initial deposition of the fertilized egg by its mother. Inside the pupal case, the...important to isolate females from males while they are still virgins. Females begin mating ~6 hours after hatching... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. Additional Resources...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently... might be for you. This methodology relies on versatile Type IIS restriction enzymes. These cut outside...mix. Nonetheless, Gibson assembly is a highly versatile and efficient way to generate a wide variety of... is extremely efficient due to the use of the versatile type II restriction enzymes. In Golden Gate cloning...

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

...developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions...instance, the DBD of the fusion protein shown is still able to bind to the upstream activation sequence...has occurred. Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and...bait and prey protein fusions to demonstrate the utility of the Y2H system, it is more common to use many...

...jellyfish for millions of years; however, it took until the 1960s for scientists to begin to study GFP and...more about GFP, how scientists have evolved this versatile protein to suit their experimental needs, and ...A. Victoria and expressed in any organism while still maintaining fluorescence. The protein structure,...mainstay in molecular biology. Scientists can easily utilize GFP-containing plasmids as a means to many functional...

...husbandry and ability to produce abundant, externally fertilized, transparent progeny (Figure 2). These features...regulate processes from development to metabolism to fertility. Several zebrafish-specific gene expression databases...screens produced ~1,500 zebrafish mutants that are still used in labs across the globe (Nüsslein-Volhard ...tracing Over a period of 24 hours, the externally fertilized zebrafish zygote transforms into an organism ...

... and put it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult)....diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming with CRISPR/...

...2019) If you’ve taken the time to do this and are still struggling with idea generation, try this thought...with an out-of-the-box concept and pare it down until you have something tangible and applicable to your...to the same problem. If your lab/company/team is still working towards a culture of encouraging dissent...easier to work with than nothing at all - they’re still ideas, after all, and that is the goal of this exercise...

...CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and is a global transcriptional ...rescue the neurological defects of Rett syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett... . https://doi.org/10.1016/j.omtm.2017.04.006 Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J...398–401 . https://doi.org/10.1038/nature24058 Tillotson R, Bird A (2020) The Molecular Basis of MeCP2 ...

..., there still remains the problem of prevention. Asbestos exists pretty much everywhere, still, in old...mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the hope is that...United States and many other countries, and it is still imported, exported, and used throughout the world...

...the Brian Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site ...conducted multiple rounds of directed evolution until they identified a mutant with significantly increased...cells. To circumvent these issues, the Ting lab utilized yeast display-based directed evolution to generate..., scientists engineered a mutant BirA* from B. subtilis, termed BASU. BASU exhibits 1,000 fold faster ...

...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...in-lab filming and remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth...