We narrowed to 1,073 results for: TOR;

...particles, particularly Resistance or R-factors. Like F-factors, R-factors could be transferred between bacteria...noted that, unlike F-factors, the evidence did not support the idea that R-factors could integrate into...cloning vectors such as pBR322, pACYC, and pUC were developed to provide higher copy number vectors that ...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...were working to understand genetic cytoplasmic factors that could be transferred between cells. At the...and the labels applied to them were vague or contradictory, owing in part to the fact that very little ...little was known about the role these factors played within an organism.  So how did plasmids get their name...

... bank account, business cards, incorporation, laboratory lease, etc. I still flip to that page in my lab...GMO food plants. When a press piece ran on the detector plants, we expected to see that same anger. Instead...business. We ended up applying to a business accelerator freely stating that we had an idea, knew how ...– $30,000 for research and another $30,000 in mentorship and business training. The money is a drop in...rough day, and we have great partners in this accelerator program who want us to succeed. We’re excited...excited to see what the next few months have in store for us. If you’re thinking about making the switch from...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside...

...minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize pairs of specific sequences...in the expression of receptors, post-translational modifications, and other factors that AAV utilizes to...in optimizing DIO/FLEx vectors is to know the recombination rate of the vector you are using. Contact ...supplier of your vector, to obtain this information.  Optimize titer for the DIO/FLEx vector Once you have...Conclusion Using AAV vectors in your experiments requires a full understanding of vector performance in your...that there are many known and unknown factors that affect vector performance in each and every system ...recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors. Proc Natl Acad Sci...

...The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer Receptor Exclusively...DREADD receptors need to be located on the plasma membrane to be activated by the DREADD actuator after...brain cells. Since the opsins or chemogenetic receptors used in these techniques cannot be directly visualized...of interest. In our studies, we used AAV viral vectors to express hM4Di in neurons of rhesus monkeys or...identify the expression of the opsin or chemogenetic receptor. However, in some cases the fluorescent signal...fluorescent protein with the opsin or chemogenetic receptor, through an IRES or 2A sequence. In this case,...subcellular compartment as the opsin or chemogenetic receptor. This may not be an important limitation in many...

...during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing DNA: temperature...way to store and elute the DNA. References Murakami M (2013) Evaluation of DNA Plasmid Storage Conditions...Figure 1: So many choices! What to do? The main factors that can affect DNA stability in solution are pH...and it will impact the time you will be able to store your sample without having substantial degradation...This is because divalent cations are essential cofactors for many tools used in modern molecular biology...things when you elute your DNA: where you plan to store it and for how long. The most common solution is...

...cytosine base editors (CBEs) and base changes from A•T-to-G•C are mediated by adenine base editors (ABEs). ...David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying...expand their precision editing capabilities. Base editors make specific DNA base changes and consist of a...change without a DNA double-strand break. But base editors aren’t perfect. They may be slow, can only target...this month. Another issue that arises for base editors is that the editing domain must induce the base...compatibility to Cas homologs. Evolving better base editors with the help of phages! The latest ABE from the...bacteria links the expression of gene III to base editor activity and editing speed and consists of the ...

... contributed  by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of... plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we hope researchers ... For many years, we’ve hoped that large scale laboratory-based functional experiments that classify which...and cancer phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge..., we’ve developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to ...Collection). In addition, we worked with many collaborators to aggregate a sequence-confirmed series of ...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

...passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame...world choose to share their plasmids through our repository since we know biological resource centers, including...+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing, we wanted to...requested plasmids. Currently, there are roughbly 400 depositors worldwide who have one or more blue flame plasmids...will serve as badges of honor for our powerhouse depositors who have generously shared their reagents freely...community. Sharing is caring We’re thrilled that our depositors reap the benefits of sharing plasmids with us...us. One way we can gauge if people are happy depositors is if they deposit plasmids more than once. People...

...blog Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We... of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous...To make sure that adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies...should you care about viral quality control? AAV vectors are produced by scientists - not robots. Unfortunately...are especially important when producing multiple vectors in parallel because the risk of accidental errors... or the cell genome can be packaged inside the vectors during production. These impurities cannot be removed...generally considered innocuous for research-grade vectors, we’d like to make sure they are present at a very...

...Co-founder/director of the non-profit foundation IMFAHE and its International Mentoring Program Director of Strategic...experience co-directing Mentor Circle Programs at BWH and providing career mentoring to several students ...successes. Although all mentorship relationships are valuable and highly recommended, a mentor with international...International Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have...to their direct training role as mentors to Spanish mentees, mentors also facilitated the development ...Impact of the International Mentorship Program and the International Mentorship Conference In addition to...international mentoring conference held at the University of Valencia (Spain). At this conference, mentors and ...

...the sample for entry into Addgene’s repository. For long-term storage in our facilities, every sample is...used for long term storage (one is kept at Addgene and another is sent to a storage facility). The third...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...sample can be tracked easily at each step in the storage process. After the original sample is barcoded,...different types of samples at Addgene - DNA sent from depositors, bacterial glycerol stocks, prepped DNA ready...

...expression vectors for insertion of custom gRNA target sequences.... CRISPR Empty gRNA Vectors CRISPR Plasmids...Plasmids: Empty gRNA Expression Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires you .... Addgene has a large collection of empty gRNA vectors. Explore our most popular plasmids for expression...tables below. Looking for non-targeting control vectors? Search our catalog for control gRNAs . All-in-...All-in-one Plasmids (gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return ...Return to top gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return to...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type II ...technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly convert bases without creating...The first base editors converted cytidine to thymidine; newly engineering base editors convert adenosine...various CRISPR-associated (Cas) genes to not only store a record of invading phages but also to destroy ...two large classes based on the structure of the effector complex that cleaves the genomic DNA. Type II ...adenosine to guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA...

...Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector Core...AAV vectors to the academic research community. The Penn Vector Core produced viral-based vectors for ...Addgene's viral vectors See Addgene's Current AAV inventory , which includes all viral vectors transferred...Transfer The University of Pennsylvania Vector Core (Penn Vector Core) and Addgene previously partnered...a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors have been...See the list of discontinued viral vectors . The following vectors are now available for distribution ...Optogenetics Baljit Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes...

...some good news with you: with contributions from depositors around the globe, we’re now sharing over 100,...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, ...

...relies on the restoration of a frame-shift in mCherry that restores fluorescence to monitor APOBEC-Cas-9...with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for the BFP-to-GFP conversion and to target...Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR...editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing in real time...time The Harris lab created ACE, a reporter that monitors APOBEC (a family of cytidine deaminase proteins...that disrupts fluorescence due to a frameshift. Restoration of fluorescence can only occur through editing...results in two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can then be sorted using...

...including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and...therapeutic potential for the split base editors. Find the split base editors at Addgene. Levy et al., Nature ...voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators (GEVIs)...broad use. This kit is designed for use with acceptor vectors from the MoClo Toolkit from Sylvestre Marillonnet...of Phytochrome B interacting factor 6 (PIF6APB), respectively. The cofactor analog, phycocyanobilin (PCB...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...sign up here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are ...

...you out. What is the Open Repository of CRISPR Screens? The Open Repository of CRISPR Screens (ORCS) database...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...research. Fortunately, there’s a growing open repository of CRISPR screen data that might be able to help... resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to...biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot...Screenshot updated 2024.   Using the Open Repository of CRISPR Screens BioGRID ORCS currently houses over ...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...

...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and...reading a publication describing how short term storage on dry ice can shift sample pH, we wondered whether...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer...freezer and then moved them to a closed dry ice storage container for 48 hours (to replicate how our viruses...from the dry ice). After 48 hours of CO2/dry ice storage, we then thawed the samples at room temperature...

...or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors allow a tight ...neuronal function directly such as receptor subunits or neurotrophic factors. Another option uses optogentics...is cloned into a viral shRNA expression vector and the vector particles are delivered into the brain region.... On the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can hold...around 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious...using viral vectors By modulating neuronal gene expression and activity, you can use viral vectors to study...viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors Resources...