We narrowed to 152 results for: Tore

1. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ...time you want to bake a cake. Instead, you go to a store and purchase flour. However, buying a bag of flour...

2. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...should be divided into single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing...

3. Fluorescent Protein Guide: Subcellular Localization

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   Collection

   ... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   Blog Post

   ...all types of data, which enables researchers to store everything in one place. From pictures to excel ...

5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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   Blog Post

   ..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   ...membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

   ...doi.org/10.1038/nbt.1791  Deal CE, Balazs AB (2015) Vectored antibody gene delivery for the prevention or treatment...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins...

9. Plasmids 101: Biotinylation

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   Blog Post

   ...consists of a biotin molecule attached to a photoreactive arylazide group. Under strong visible light,...

10. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ...) to name two more. Likewise, there are several store-bought cloning kits available which are all based...

11. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...is engaged in a temporary and defined period of mentored advanced training to enhance the professional ...

12. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond to specific wavelengths...

13. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...and repair pathways work downstream of Tet1 to restore the DNA after cytosine removal. Recently, Albert...

14. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene...

15. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to...

16. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...Kenneth W., et al. "A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote ...

17. Quick Guide to All Things Lentivirus

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    Blog Post

    ...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

18. Antibodies 101: Validation

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    Blog Post

    ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

19. Genetic Code Expansion

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    ...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...

20. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    ...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...