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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 770 results for: VARS

Showing: 101 - 120 of 770 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...results in a heterogenous polyclonal population that varies in the number of integration events and the site...culture has, in theory, been transduced. There are various schools of thought on using selective medium throughout...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

  2. CRISPR References and Information

    Type
    Collection
    ...Toolkit) (Link opens in a new window) A library of various python functions for the purpose of designing and...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...several sources (Chan Zuckerberg Initiative, MedGen, ClinVar) and is meant to be representative but not exhaustive...DNMT1 Hereditary sensory neuropathy type IE Paul Khavari 25044 pDEST53-LRRK2-WT LRRK2 GFP CMV Parkinson'...pCB268 APP Flag, HA, Myc CMV Alzheimer's Alexander Varshavsky 53810 SYNJ1-145_pCSdest SYNJ1 CMV, SP6 Parkinsonism...Hereditary diffuse leukoencephalopathy Markku Varjosalo 187827 pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham N, Cristiano C, Dorrier CE, Tipton GJ...

  5. The Pleiades Promoter Project

    Type
    Collection
    ...Swanson, M. I., Tam, B., Ticoll, A., Turner, J. L., Varhol, R., Vermeulen, J., Watkins, R. F., Wilson, G.,...

  6. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...vector DNA and insert DNA used in the ligation will vary depending on the size of each and their concentration...failed ligations. The following table indicates the various controls: Control Ligase Interpretation Uncut vector...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...recombinant antibody can be purified for use in a variety of applications. Sharing speeds science. We believe...to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should be empirically determined...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...protocol. Sample Data Figure 1: HEK293T cells at various confluencies. This AAV production protocol should...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637 NS2B C 79636... activity have remained challenging due to the variable availability of enhancer sequences in different...the entire genome for genes that regulate a wide variety of phenotypes, including but not limited to cell...CIDAR MoClo) Parts Kit. This Kit consists of a variety of plasmids containing many different promoters...RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined with your...creation of many E. coli constructs with greatly varied levels of expression. The 93 plasmids in the CIDAR...gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts...

  11. Water Bath Protocol

    Type
    Protocol
    ...in your protocol. How to set the temperature will vary depending on the model of water bath, but will likely...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...functions, T4 DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific...

  14. Video Library

    Type
    Protocol
    ...Scientific Project Leader Eric Perkins to discuss the various positions he has held in his career, his favorite...

  15. Centrifugation

    Type
    Protocol
    ...Depending on the centrifuge their speed ranges can vary, but are typically lower than some other centrifuge...

  16. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...Independent variables – factors which are not affected by other variables (cause). Dependent variables – factors...assumptions: Distribution – Normal Variables – Independent Variance – Approximately equal Data type - ...two (two-way ANOVA) independent variables on a single dependent variable. A two-way ANOVA could test the... the effect of two dependent variables on up to two independent variables. For example, a one-way MANOVA... assesses multiple independent variables on a single dependent variable. I know what you’re thinking –...and MANCOVA All variants of ANOVAs discussed so far assume that the independent variables are independent...have discrete effects on the dependent variable, and those variables are the only things that matter. However...

  19. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ...individual variants found in tumors are exceedingly rare. As a result, how most of these “variants of unknown...of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine...cancer clinical sequencing report includes rare variants that haven’t been characterized? Some have developed...approach this challenge, using attributes of cancer variants such as evolutionary conservation, or predicted...hope researchers can use to characterize these variants in a high-throughput manner and therefore push...precision medicine a reality. Challenges for cancer variant characterization For many years, we’ve hoped that...laboratory-based functional experiments that classify which variants are likely gain-of-function, loss-of-function ...

  20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the use of Cas9 homologs...four novel SpCas9 variants with altered PAM binding specificity: SpCas9 variant Mutations (relative...SpCas9) PAM sequence D1135E variant D1135E NGG VQR variant D1135V, R1335Q and T1337R NGAN...NGAN or NGNG EQR variant D1135E, R1335Q and T1337R NGAG VRER variant D1135V, G1218R, R1335E...the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants are likely just ...reported the development of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs...R1335E and T1337R NGCG The D1135E variant is far more selective for the canonical S. pyogenes PAM...
Showing: 101 - 120 of 770 results