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We narrowed to 221 results for: acr.2

Showing: 101 - 120 of 221 results

  1. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...identifying the resources needed to accomplish that goal. 2. Identify the tools in that timeline Avoid writing...based on experimental timelines and specific aims. 2. Then make decisions based on financial values. All...Would I be able to get access to these materials?” 2. Always negotiate for a win-win decision An institute...early investigators making unnecessary short term sacrifices that hurt their goals, rather than focusing on...

  2. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...scientists have 1) the courage to make mistakes and 2) the integrity to recognize these mistakes quickly...She studies beta-cell function and failure in Type 2 Diabetes. Birmingham, AL, USA 33.5207°N 86.8025°W ...available to people who want to join the dialog and 2) remember that science should not be partisan: everyone...nightmare that is Times Square. But I made the sacrifice in April because devaluing science has alarming...reinforce their scientific interests. Our marches across the world have had an impact already, gaining additional...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...of ~75% in response to the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled...didn’t need any added cofactors.      Figure 2: Fluorescence intensity and ΔF/F of MagLOV, a variant...sample. The imaged area measures approximately 82 mm across. Video courtesy of Andrew York and Maria Ingaramo...new microscopy tools for biological applications across a wide range of topics. He embraces open science...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection...Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I ...because it can be a one-stop shop for selection across systems and species. The cost? Zeocin is genotoxic...off-target mutations are undesirable. Pros: Works across many species/systems. Cons: Doesn’t work for all...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells, including...(#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  6. Hot Plasmids - January 2023

    Type
    Blog Post
    ...is always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid...the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids...cytoplasmic mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. ...

  7. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad... Yamanaka explained that it took 50 people nearly 2 years to create the first stem cell line that could...cells, and the difficulty of sharing banked iPSCs across international borders. While questions of ethics...

  8. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...misplacing your results or losing that lab notebook from 2 years ago. The free subscription to Lab Archives allots...notebooks you’re saving trees! The Earth thanks you. 2. Gibco Cell Culture by Gibco Whenever I start to learn...could help me with calculations. This is how I came across the Gibco Cell Culture app. Gibco organizes its...

  9. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441-2. PubMed. Find the plasmids from this publication ...Levskaya A, Voigt CA. J Mol Biol. 2011 Jan 14;405(2):315-24. doi: 10.1016/j.jmb.2010.10.038. Epub 2010...this far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded...

  10. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted in plasmid...matching!   Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to...debris”; and then clean-up the plasmid DNA (Figure 2). For most kits, the steps for lysing cells and removing...

  11. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 ...PMID: 24086725. PubMed Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher...patterns of transduction and transgene expression across different cell types (Aschauer et al., 2013). Expression...

  12. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...animal is given additional booster immunizations every 2-3 weeks to increase the antibody titer. The titer ...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of...antibody, you’ll want to use the same positive control across experiments to be aware of signal strength. In ...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral...Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016...about a third of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The...

  14. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ... engineered components, like protein tags (Figure 2). Through the work of many groups, there are systems... in Addgene's repository (Table 1). Figure 2: Adding function to My Favorite Protein (MFP) through...— Click Biology tags should be similarly useful across the diverse kinds of proteins that one finds in...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and...pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications. Like the TALE-LSD1...approach for targeted DNA demethylation. Cell Discovery 2: . https://doi.org/10.1038/celldisc.2016.9 Find plasmids... epigenome (the sum of epigenetic modifications across the genome) has been implicated in disease states...

  17. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...immediate early gene expression. Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101/gad.2.6.718. PMID: 2843425...Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID: 20697123. PubMed Central... in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153. Additional... gene expression and tracing of gene expression across development. One common binary system is the GAL4...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... reduce the background staining (Table 2).      Table 2: Secondary antibody selections for a four-antibody...commercially available secondary antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...days before the deadline isn’t going to be much use. 2. Originality is essential for grant success The two... it’s important not to throw in the towel if 1 or 2 funding bodies say no. If possible, get feedback from...you be interested in it?) so make sure that comes across. Check out some in-depth grant writing tips at ...

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...due to the presence of additional helper sequences (2-3). As alluded to above, termination and polyadenylation...and translation processes as well.   Figure 2: The conserved eukaryotic polyadenylation signal directs...The purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves...
Showing: 101 - 120 of 221 results