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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 210 results for: acr.2

Showing: 101 - 120 of 210 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...of ~75% in response to the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled...didn’t need any added cofactors.      Figure 2: Fluorescence intensity and ΔF/F of MagLOV, a variant...sample. The imaged area measures approximately 82 mm across. Video courtesy of Andrew York and Maria Ingaramo...new microscopy tools for biological applications across a wide range of topics. He embraces open science...

  2. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection...Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I ...because it can be a one-stop shop for selection across systems and species. The cost? Zeocin is genotoxic...off-target mutations are undesirable. Pros: Works across many species/systems. Cons: Doesn’t work for all...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...is always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid...the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods   Find YFP plasmids...cytoplasmic mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. ...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells, including...(#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad... Yamanaka explained that it took 50 people nearly 2 years to create the first stem cell line that could...cells, and the difficulty of sharing banked iPSCs across international borders. While questions of ethics...

  6. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...misplacing your results or losing that lab notebook from 2 years ago. The free subscription to Lab Archives allots...notebooks you’re saving trees! The Earth thanks you. 2. Gibco Cell Culture by Gibco Whenever I start to learn...could help me with calculations. This is how I came across the Gibco Cell Culture app. Gibco organizes its...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441-2. PubMed. Find the plasmids from this publication ...Levskaya A, Voigt CA. J Mol Biol. 2011 Jan 14;405(2):315-24. doi: 10.1016/j.jmb.2010.10.038. Epub 2010...this far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted in plasmid...matching!   Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to...debris”; and then clean-up the plasmid DNA (Figure 2). For most kits, the steps for lysing cells and removing...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 ...PMID: 24086725. PubMed Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher...patterns of transduction and transgene expression across different cell types (Aschauer et al., 2013). Expression...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...animal is given additional booster immunizations every 2-3 weeks to increase the antibody titer. The titer ...level of antibodies is reached - typically within 2-4 months. After the animal has the desired level of...antibody, you’ll want to use the same positive control across experiments to be aware of signal strength. In ...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral...Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016...about a third of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and...pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications. Like the TALE-LSD1...approach for targeted DNA demethylation. Cell Discovery 2: . https://doi.org/10.1038/celldisc.2016.9 Find plasmids... epigenome (the sum of epigenetic modifications across the genome) has been implicated in disease states...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...immediate early gene expression. Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101/gad.2.6.718. PMID: 2843425...Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID: 20697123. PubMed Central... in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153. Additional... gene expression and tracing of gene expression across development. One common binary system is the GAL4...

  15. Optogenetics Guide

    Type
    Guide
    ...influx into the cell when stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool,... (Phy) interaction 660 Cryptochromes Cryptochrome 2 and CIB1 Blue-light–mediated induction of protein ...interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 450 LARIAT Inhibits protein function by reversibly...customizable TALE DNA-binding domain with cryptochrome 2 protein and its interacting partner CIB1 to control...transcription effectors OPTOSTIM PHR domain2 of cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated... with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185 Kim B, Lin MZ. 2013. Optobiology...comparative analysis of microbial opsins. Nat Methods. 9(2):159-72. PMID 22179551 Saunders A, Johnson CA, Sabatini...

  16. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... reduce the background staining (Table 2).      Table 2: Secondary antibody selections for a four-antibody...commercially available secondary antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    ...days before the deadline isn’t going to be much use. 2. Originality is essential for grant success The two... it’s important not to throw in the towel if 1 or 2 funding bodies say no. If possible, get feedback from...you be interested in it?) so make sure that comes across. Check out some in-depth grant writing tips at ...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...due to the presence of additional helper sequences (2-3). As alluded to above, termination and polyadenylation...and translation processes as well.   Figure 2: The conserved eukaryotic polyadenylation signal directs...The purpose and mechanism of polyadenylation vary across cell types, but polyadenylation generally serves...

  19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...Hanno, et al. "Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine...can quantitatively determine allele frequencies across timepoints or populations. This kit consists of...

  20. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ..., and the desired gene (transfer vector plasmid), 2) the HIV-1 genome containing all viral genes with ...protocols that have been described (protocol 1, protocol 2, protocol 3) to find the one that will give you the...PubMed PMID: 3785217. PubMed Central PMCID: PMC367857. 2. Naldini, Luigi, et al. "In vivo gene delivery and...quiescent or terminally differentiated cells such as macrophages and microglia. This property makes the lentivirus...
Showing: 101 - 120 of 210 results