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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 398 results for: aga

Showing: 101 - 120 of 398 results

  1. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...abilities, we’re gaining new ground in the fight against cancer in its many forms. The dichotomous interactions...sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...will more broadly activate the immune response against tumors. Some chemotherapeutic agents induce immunogenic...

  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Distribution Please indicate which strain Addgene should propagate your plasmids in: DH5α, NEB Stable, or ccdB Survival...Survival.­ Whenever possible, we propagate plasmids in the standard cloning strain DH5α. Please indicate...indicate if your plasmid cannot be propagated in DH5α. For plasmids with highly repetitive sequences (which...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...approach, which focuses on validating antibodies against markers of the human blood leukocyte populations...on human blood leukocytes, including antibodies against these markers. The 371 CD markers characterized..., and include information on tested antibodies against the target. Helpfully, this also includes clone...

  4. CRISPR Guide

    Type
    Guide
    ...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

  5. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...scientists delivered some spectacular photos once again this year! All winners receive an Addgene care package...(@ChiTownBio) and one of our favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came...

  7. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...ladders, the 100 bp ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying... to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel Stains on ...

  8. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...impressive science-themed holiday decorations yet again. This year, we had two different categories: one...his Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This ...

  9. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...Court’s decision to ban gene patents in a case against Myriad, an organization called Free the Data launched...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... antibodies drive an effective immune response against a pathogen. The Fc domain creates a link between...Inhibitory Fc receptors modulate in vivo cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi...

  13. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...

  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial...

  15. RUBY-Red Siliques

    Type
    Blog Post
    ...applications. But some techniques require you to select against the presence of a transgene instead, which can’...are currently used in these situations to select against CRISPR positive plants, but screening these can...

  16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...THE RESULTS DISCUSSED BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...

  17. Plasmid Cloning by PCR

    Type
    Blog Post
    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  18. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...lost for an age) fight in the Napoleonic Wars and against something even more sinister. 1000 pages long. ...reads, some are hard. Some I’ll never think about again, some I loved so much I’ll force them on everyone...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...
Showing: 101 - 120 of 398 results