We narrowed to 568 results for: bli
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TypeBlog Post...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...
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What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...have the same blind spots, and there wouldn’t be anyone to question these blind spots. Blind spots often...result of our diversity which in turn minimizes the blind spots in our work. Agile at Addgene We follow an... -
Tips for Getting a Faculty Position
TypeBlog Post... do need multiple first/corresponding author publications in quality journals. It helps to come from the...a job. The currency of scientific success is publications and grants. 2. Write succinct and easy to understand...what I was proposing. The result of my test was humbling. My conviction that I had a flair for communication.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi...and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W,... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...Caltech’s CLOVER Center’s extensive repository of published systemic AAV experiments and Addgene’s AAV Data...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...are reusable, saving time and money on future assemblies. If you are new to MoClo, give our MoClo 101 ...save time and money on studies that require many assemblies or that compare the effects of different DNA ...PLoS ONE, edited by Jean Peccoud, vol. 6, no. 2, Public Library of Science (PLoS), 18 Feb. 2011, p. e16765...PLOS ONE, edited by Alvaro Galli, vol. 13, no. 5, Public Library of Science (PLoS), 30 May 2018, p. e0197185... -
Developing Transferable Skills During Science Training
TypeBlog Post... with another lab, in another field maybe, and publish Join a lab that has multiple collaborations, even... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend... for Researchers called Vitae. Vitae recently published a career development framework for career skills... -
Why Do I Need an MTA Anyway?
TypeBlog Post... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...applications on modifications or inventions Right to publish without editorial comment or review by the Provider... -
Management for Scientists: Seeking Feedback
TypeBlog Post...ask direct questions for specific feedback in non-public settings. Ask over coffee. Have regular 1:1 meetings...information to make change whenever possible. Be public about changes influenced by feedback. If you don't...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...2):131-3. PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene. Addgene’s Fluorescent Protein... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...Base editing publication highlights In the table below, you'll find a list of publications we've highlighted...editor was named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10...highlighted in this article. Publication Plasmids Highlights Komor et al., 2016 BE1, BE2, BE3 BE3... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post... have access to through your university or your public library. If you want to use pre-drawn images in...without the watermark, which are better suited for publications, if you sign up for a paid account. I’m proud...easier to give attribution, than for journal publications. Google Image Search Google image search is ... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post...c)(3). That means we were formed to benefit the public, not private interests. On this nonprofit awareness...initiatives as opposed to fundraising. Aside from enabling us to make all decisions with the goal of benefiting... well. We work every day to do a better job of enabling more scientists in labs all over the world to ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...arrange permits to visit places not accessible to the public and retrofitting our car with a freezer wired to...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...conditions. One example application from the publication is the removal of unwanted cDNA derived from ... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...science is much like industry but the “product” is publications—the pressure to produce is the same You get ... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary...is different about non-academia? Scientific publications are a nice to have, not a need to have Must ... -
Intro to Our Summer SciComm Series!
TypeBlog Post...interested public.” Subscribers to Scientific America would be considered the interested public and it would...podcasts A series of Tweets breaking down a new publication that could advance treatments for a rare disease... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo ...PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al. “DNA-... PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post... of print] PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9...2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and versatile genome editing...2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional Resources on the Addgene... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...she would be looking at where I had published, who I had published with and whether I would be able to...blogger! Seán was a PhD student at University College Dublin & a post-doc a Cambridge University where he studied...