Addgene

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Showing: 101 - 120 of 310 results

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Plasmid Preps: Different Purity, Different Quantities, Different Uses

Type

Blog Post

...cloning of your gene of interest into a plasmid backbone. Transfection grade DNA Figure 3: Need...
Your Top Requested Plasmid in 2015!

Type

Blog Post

... genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus...
What's New in CRISPR - Spring 2019

Type

Blog Post

...reaction. STAgR is highly customizable as vector backbones, and gRNA structure, numbers, and promoters can...
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

Type

Blog Post

... ready-to-use KTRs for p38, JNK, and ERK, or a backbone to use for custom KTR design, plasmids are available...
Addgene's Top Blog Posts from 2020

Type

Blog Post

...service. This service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids...
Editor's Choice, September 2016

Type

Blog Post

...opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our September posts in...
Top 10 Open Science Developments of 2013

Type

Blog Post

...published systematic reviews on an agent used to promote bone growth based on data voluntarily made available ...
Searchable and Sortable gRNAs for Your Next CRISPR Experiment

Type

Blog Post

...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs...
Seeing Red: Simple GFP Photoconversion

Type

Blog Post

...for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et...
Inside Addgene's 10 Year Anniversary Gala

Type

Blog Post

...thing was clear: the sense of family is still the backbone [ba-dum-chh] of this crazy group of Addgenies....
Antibodies 101: Affinity Tags

Type

Blog Post

...six consecutive His residues (or more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper...
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

Type

Blog Post

...information contributed by the depositor, published backbone sequences, and any known sequences for the insert...
SunTag and Fluorescent Imaging

Type

Blog Post

...on Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find ...
Sleeping Beauty Awakens for Genome Engineering

Type

Blog Post

...flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid contains the transposase gene...
CRISPR/Cas9 FAQs Answered!

Type

Blog Post

...designing oligos for cloning my target sequence into a backbone that uses the human U6 promoter to drive expression...
Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

...our Plasmid Protocols Find Your Perfect Plasmid Backbone ...
History of CRISPR Cas - A tale of survival and evolution

Type

Blog Post

...Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. Nature...
Viral Vectors 101: What is a Virus (and a Viral Vector)?

Type

Blog Post

...to a cell but cannot create new particles. As a bonus, this increases the carrying capacity of the viral...
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Type

Blog Post

...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions ...
Plasmids 101: Origin of Replication

Type

Blog Post

... base pairs are held together with two hydrogen bonds not three as G-C pairs are. As a result, stretches...

Showing: 101 - 120 of 310 results

Results per page:

Plasmid Preps: Different Purity, Different Quantities, Different Uses

Your Top Requested Plasmid in 2015!

What's New in CRISPR - Spring 2019

Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

Addgene's Top Blog Posts from 2020

Editor's Choice, September 2016

Top 10 Open Science Developments of 2013

Searchable and Sortable gRNAs for Your Next CRISPR Experiment

Seeing Red: Simple GFP Photoconversion

Inside Addgene's 10 Year Anniversary Gala

Antibodies 101: Affinity Tags

Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

SunTag and Fluorescent Imaging

Sleeping Beauty Awakens for Genome Engineering

CRISPR/Cas9 FAQs Answered!

Plasmids 101: Antibiotic Resistance Genes

History of CRISPR Cas - A tale of survival and evolution

Viral Vectors 101: What is a Virus (and a Viral Vector)?

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Plasmids 101: Origin of Replication

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