We narrowed to 102 results for: cas9 expression construct

...target non-dividing cells, enable stable long-term expression in a host, and exhibit low immunogenicity. This...second-generation packaging system because transgene expression from the LTR is Tat-dependent. Figure 3: Second-generation...) can be used in transient transfections, but expression will be greatly limited due to the LTRs. Generally...be used in direct transfections for transient expression of a transgene. For more detailed protocols, ... VSV-G is toxic under long-term, constitutive expression. In order to make stable cell lines, VSV-G is...Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are useful...fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More...

...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...showed that AAV-delivered CHARM could silence the expression of prion protein in mouse brains for at least...gene product; it simply turns the target protein expression off. Plus, DNA methylation is a long-lasting ...-silencing CHARM variants that stop their own expression after silencing their target — potentially improving...by targeting marker proteins that are uniquely expressed in a given cell type. For example, glial fibrillary...GFAP) is a glia-specific intermediate filament expressed in astrocytes throughout the brain, making it ...Fourier-shell correlation curve (D), and the reconstructed 3D map with 3.1 Å global resolution (E). Figure...