We narrowed to 200 results for: ckb

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

4. Making CRISPR Plasmids Using Fragmid

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   ... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

5. Hot Plasmids and Viral Preps - March 2021

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   ...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

6. How-to: 5 Steps to a Great Panel Discussion

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   ...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

7. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

8. Plasmids 101: Optimizing Plasmid Yields

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   ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you...

9. Plasmids 101: Multicistronic Vectors

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   ...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

10. Tips for Using BLAST to Verify Plasmids

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    ...sequence, check the “Align two or more sequences” checkbox and paste your reference sequence into the Subject...

11. Simplify Cloning with in vivo Assembly

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    ...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...-White Screening Choosing Your Perfect Plasmid Backbone  ...

13. Antibodies 101: Producing Recombinant Antibodies

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    ...Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports...

14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    .... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...

15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription...

16. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

17. Using Video to Share Your Science: We Share Science

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    Blog Post

    ...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,...

18. Viral Vectors 101: Preparing Pooled Libraries

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    ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

19. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    .... These sequences were then cloned into an AAV backbone containing a minimal promoter to drive expression...

20. Plasmids 101: Shuttle Vectors

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    ...Resources on Addgene.org Collection of empty plasmid backbones ...