We narrowed to 428 results for: cript

...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...’t give tRNAs much thought. High throughput transcriptome sequencing methods including work done by the...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

...histone modifications, which can influence the transcription of genes by RNA polymerase II, or by the addition...methylcytosine in CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying...of tumor suppressor genes can repress their transcription and inactivate them. Global hypomethylation,...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...vivid chromatin picture, CAPTURE 2.0 re-writes the script on gene regulation. References Han, J., Zhang...

...Unlike promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic...your gene as they are both derived from the same transcript. Of course multicistronic vectors do not have...efficient translation of multiple genes from a single transcript. The two strategies most widely incorporated ...

..., kinase signaling, integrin signaling, and transcription factor interactions (Kerppola, 2006). One of...interactions between basic leucine zipper and Rel transcription factors (Hu et al., 2002). This would be more...frame (ORF) library to screen PPIs among 450 transcription factors in live fruit flies. Although BiFC open-reading...

...backbones for constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a...promoter in a given cell type. This type of transcription reporting was among the earliest uses of GFP... gene of interest, or expressed from the same transcript as the gene of interest, but after an internal...

...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...

...protease allows a transcription factor to enter the nucleus and modulate transcription of the mCitrine ...activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed ...

...we probably exchanged dozens of drafts of the manuscript. This is the time to transform the document into...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out...

... with tRNAs, which are cleaved out of the RNA transcript by endogenous processing enzymes (Figure 1). ...processing only occurs on the RNA polymerase III transcript, the sgRNAs were encoded on the lentiviral minus...including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of host cell proteins, and...

...developed a separate script for manually inputting known innocuous differences. This script compares the sequence...Any sequences that are a perfect match from this script can then continue in the auto-align process. This...

...specialized strains are available to confer greater transcriptional control, assist with proper protein folding...Lacks functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm... the expression plasmid in order to repress transcription from a hybrid promoter. Although the DE3/T7 ...

...policy and recently announced that of the 16,000 manuscripts that had been processed since the declaration...access of this data is to be made at the time of manuscript submission. The rationale includes increasing...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

...amplified. RPA reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification is...thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the amplified...First, RNA is extracted and subject to reverse transcription. This is then amplified by PCR using Taq Polymerase...

...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

... editor or adenine base editor) and reverse transcriptase-mediated prime editing technologies have been...Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https...CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 ....

...pathological condensates, including oncogenic transcription, proliferation of leukemia cells, and assembly...easily visible chromoproteins in a cell-free transcription-translation system (Figure 6). This approach...CRISPRkit uses a cell-free system to carry out transcription and translation of chromoprotein and gRNA plasmids...

...Antibody-independent methods may include analysis of transcriptomic or proteomic data from several different tissues... feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want...tissues. You know that there is a wealth of transcriptomic data from this sample type, so you use your...