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We narrowed to 437 results for: ena

Showing: 101 - 120 of 437 results

  1. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells. More broadly,...lentiviral gRNA expression vector (pRDA_526) that (1) enables efficient knockout (KO) of genes in immune cells...also includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen, Ovalbumin, into...

  2. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...university partners quickly assessed how to more readily enable research. Often “normal” timelines that could be...resource from the Structural Genomics Consortium enables science to determine the three dimensional structures... proteins.  The RESOLUTE Consortium collection enables research on solute carriers (SLCs) for medical ...

  3. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...ways in which we promote our mission and work to enable researchers around the world. We take this responsibility...initiatives as opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting... well. We work every day to do a better job of enabling more scientists in labs all over the world to ...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour...by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier. PLoS Biology...carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously   Researchers...between a protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl between... to the many plant studies that are sure to be enabled by this toolkit. For examples of the toolkit in...layer of regulation to genome engineering that enables new applications, such as reversing the effects...

  8. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...sharing, computational reproducibility, and tools to enable scientific reproducibility. This workshop was well...one at a scientific conference could potentially enable reproducibility practices being adopted by a wider...the group, lessons learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Fab fragments can be used as a block in similar scenarios. By applying unconjugated Fab fragments that are...site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest...which antibody format to use for your specific scenario.    References and resources References Bates...

  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...frequently and recent advances in AAV technology have enabled us to generate new and refined forms of capsids...This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations. AAV...deeper understanding of disease pathogenesis will enable robust therapeutic outcomes. In contrast to other...

  11. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...Using these reagents and a series of heating (denaturing) and cooling (annealing) steps Taq polymerase...specifics of the 3 basic steps of a PCR reaction:  Denaturation- To amplify DNA, the two strands of the template... Steps in PCR reaction   This process of denaturation, annealing and extension is repeated 25-35 times...

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...can be calibrated to these different conditions, enabling the use of a given timer in many experimental ...protein expression dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used... 19478145. PubMed Central PMCID: PMC2712795. 6. Saxena, Pratik, et al. "A programmable synthetic lineage-control...

  13. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... Recent improvements in computer modeling have enabled researchers to begin discovering and designing ...signaling pathway. Its major appeal is that it enables the simple construction of vectors to study the... PMID: 24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID...

  14. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...considered when using this method:     Cells must be amenable to transfection or viral transduction     Appropriate...studies in experimental systems that may not be amenable to plasmid based methods. One of the most common... "Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro...

  15. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...implemented and from that, develop better software.  Maintenance  We’re not just about developing new features...: we take time during each sprint to do some maintenance work on our code. One way to look at this is ...continuing to add new features.  Another area of maintenance is agreeing to the names of the things in our...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... provide a modern and fully supported API that enables development of custom integrations with other tools.... As illustrated in this video the integration enables RSpace users to search for protocols in protocols.io.... These integrations utilize an interface that enables users to conveniently select and deposit individual...

  17. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...Principal Investigator meeting of the NSF EDGE (Enabling Discovery Through Genomic Tools) Program and I...the NSF program directors, this program should “enable the ability to study life’s innovations from anywhere...has created an automated tank for feeding and maintenance that had the audience gasping. Animal husbandry...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    ...generous donation of a Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site,...how the various pieces were connected, as this enabled us to rapidly iterate and optimize our pipeline...used Nextflow Tower to augment our pipeline and enable our team members to automate their weekly sequencing...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...standard, denaturing Western blot will not be suitable for an IP, as proteins in an IP are not denatured (learn...for an IP, samples are typically lysed in a non-denaturing buffer containing non-ionic detergents, such ...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase expression that... crRNA can be customized as well, including concatenations into crRNA arrays targeting multiple different...
Showing: 101 - 120 of 437 results