We narrowed to 257 results for: gfp gene

...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83900-AAV2 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21217 ...61803 GFP-Rab7A Endosomes (late) Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Endosomes (late) RAB7 GFP Richard...Targeting Gene/Signal Fluorescent Protein PI 26722 GFP-cortactin Actin filaments Cortactin EGFP Anna Huttenlocher...38277 pMXs-puro GFP-p62 Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes...emiRFP703 Vladislav Verkhusha 17650 GFP-HP1gamma Chromatin HP1 gamma GFP Tom Misteli 55068 mCherry-LaminA-C...118736 pBOB-CARMIL2 BH domain-GFP Plasma membrane CARMIL2 BH domain GFP John Cooper 118737 pBOB-CARMIL2...Structure Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens junctions ...

...Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein ...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden MaCPNS2 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B10 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden CAP-B22 These viral vector preparations...Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9-X1.1 These viral vector preparations...

...James Iglehart 20672 MSCV-IRES-GFP MSCV For transgene expression with a GFP marker. Tannishtha Reya 21654...plasmid — contains packaging genes Envelope plasmid — contains packaging gene env This collection highlights...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid...William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in mammalian...21654 pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin...puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression...Scott Lowe 9044 pMIG MSCV Plasmid for transgene expression with GFP expression. See plasmid 12282 for the...

...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Karl Deisseroth 58880 pAAV-Syn-ChR2(H134R)-GFP Syn ChR2/H134R GFP Constitutive 8, rg* Edward Boyden 100054...Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive 9 Edward Boyden 107712...107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent 9 Edward Boyden 35505 ...Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650 pAAV-CamKIIa-C1V1...Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Edward Boyden 59171...Constitutive 8 Karl Deisseroth 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Edward Boyden 28305 pAAV-FLEX-ArchT-tdTomato...

... time or location can be critical when, say, the gene is essential during development and a knockout is...activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series of HEK 293T cells expressing...membrane-targeted SuperPLDx30 (magenta) and PA probe GFP-PASS (green). After 30 minutes of blue light illumination...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...

...the puromycin resistance gene through a 2A peptide. The puromycin resistance gene enables selection of cells...to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This is the vector backbone...had a point mutation in the puromycin resistance gene that made it less effective in certain cell lines... lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9 and further...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...

...translational. Ex: the discovery of GFP. Chapter 4: Goals of the Center for Regenerative Meidince - Understanding...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...treat disease through tissue repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Darrell Kotton is the director of the Center for Regenerative Medicine at Boston University Medical School...source biology in his own lab, at the Center for Regenerative Medicine, and within the biomedical research...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...

...NIR FPs can be used similar to GFP-like probes by supplying a single gene to cells. Still in bacteria, ..., which do not produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et...crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova et al., 2016), as well as ...applications of fluorescent proteins, genetically encoded biosensors, and optogenetic tools. Follow her recent research...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent...FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools based on...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference...

...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control... 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent...105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control James M. Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG...Flp-dependent Optogenetics Karl Deisseroth 58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Edward ...Cre-dependent Optogenetics Edward Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CamKII Inhibitor Optogenetics Edward...Recombinases James M. Wilson 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP-tagged Cre expression Recombinases...

...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/... vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression ...destination vector for gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection...one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones made by Addgene Adenoviral High...

...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and ...further customizing gene expression. Sahoo et al. have tested the system with genes up to 4 kb in length...versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants...Scientists have worked to optimize this system for gene transfer, studying the stability of modified Ti plasmids...versatile binary Ti vector for both transient and stable gene expression applications in plants. This smaller,...and many plant scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity.... They also replaced the promoter regulating the gene of interest with a constitutive, robust M24 promoter...

...proteins (FPs) to a yeast gene of interest. These fluorescently tagged genes are expressed under native...sequence and a selectable gene product for isolation of successful transformants. Addgene distributes several...be interested in attaching epitope tags to their genes of interest, allowing for easy capture and detection...modifications in S. Pombe, including full and partial gene deletion, overexpression (by promoter substitution...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...few. Have you used these tools in your own lab? Addgene would love to hear from you, our community, about...

...biosensors To focus on a particular gene or protein, check out our gene-specific biosensors. These constructs...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin ...Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration..., and voltage across membranes. These sensors generally follow the same pattern described above - a sensing...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...

...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...

...cells - one containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA...the env gene containing the rev response element (RRE), an internal promoter, and the desired gene (transfer...-1 genome containing all viral genes with the exception of the env gene (packaging plasmid), and 3) the...used to express your gene of interest either constitutively or inducibly. If your gene of interest is toxic...expression of your gene. The addition of Dox in the culture will then repress your gene of interest. This... Youichi Suzuki (2011). Gene Regulatable Lentiviral Vector System, Viral Gene Therapy, Dr. Ke Xu (Ed.)...harmful genes into a transfer vector. Lentiviral particles expressing proto-oncogenes or oncogenes for instance...

...pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics Ed Boyden AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden...pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics Ed Boyden AV-5-PV3446 59170-AAV5 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden...AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics Ed Boyden...29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446 59170...D156A)-EYFP Optogenetics Karl Deisseroth AV-1-PV2432 22222-AAV1 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden...pAAV-Syn-ChrimsonR-tdT Optogenetics Ed Boyden AV-5-PV3638 65014-AAV5 pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden...pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden AV-9-20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel ...

...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...which come from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...in the DNA of the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you...monoclonal antibodies, recombinant antibodies are a homogeneous population. However, unlike monoclonal antibodies...antigen binding, the variable regions alone can be generated as a fusion protein. An ScFv is made up of the...

...viruses containing transgene under CMV promoter Vogelstein 16404 pAdTrack For production of GFP-trackable viruses...containing transgene under a chosen promoter Vogelstein 16405 pAdTrack-CMV For production of GFP-trackable...mRFP-trackable viruses containing transgene under CMV Bamburg 16407 pAdEasy 2-GFP beta-gal Test plasmid that ...Adenoviral Strains Adenoviral vectors are widely used for gene delivery due to their high packaging capacity, high... high efficiency in driving strong transient gene expression in both dividing and non-dividing cells, ...adenoviral vector production. Want a plasmid with your gene of interest? Search our database for shuttle plasmids...that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer Vogelstein 18703 pEZYshuttle Modified pShuttle-CMV...

...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...first-generation transposase (as determined by transposition assays of antibiotic-resistance genes conducted...). In their Nature Genetics paper, the lab showed that efficiencies of stable gene transfer using SB100x... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...transposase enables robust stable gene transfer in vertebrates. Nat Genet. 2009 Jun;41(6):753-61. Epub 2009... et al. A new approach to gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells...