We narrowed to 269 results for: gnal

...networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal...

...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

...PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which...the aptamer improves stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius...degraded by Cas12a, it will release a quantifiable signal that indicates the presence of your target DNA ...cleaves the reporter as well, and a quantifiable signal is produced, indicating the presence of that specific...Pederson, T. (2018). CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nature Methodss...Vale, R. D. (2014). A Protein-Tagging system for signal amplification in gene expression and fluorescence...

... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...

...protease cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility...purification. Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep...TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE...

...improves protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds...Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through the...

...sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single pulse...pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten pulses...

...exactly what iRFPs achieved. Those proteins allowed a signal-to-background ratio in mammalian tissues 20-fold... In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher resolution...

...material, cells or tissue, to produce a strong enough signal over background noise. This is not terribly problematic...large numbers of cells or when a high degree of signal to noise is desired. It also provides an excellent...

...amplify fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio...

...molecular level. The brain is constantly influenced by signals it receives from the entire body, making it difficult...advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were very few...

...the riboswitch results in increased fluorescent signal. Prior to the development of these probes, fluorophore...less prone to photobleaching, and achieve better signal in vivo as compared to imaging with broccoli aptamers...

...locations, measure fluorescent signals from the sensor, and send a signal including its position in the...

...displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...reporter. Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind...

...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...

... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...

...classified function and a well established cellular signaling gene, thus providing a starting point for downstream...series of clones corresponding to known cellular signaling pathways as part of our Genetic Pathway Reference...