Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 267 results for: gnal

Showing: 101 - 120 of 267 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it...single action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak...Waldron Glypicans are important modulators of cell signaling pathways during embryonic development and cancer...

  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

  3. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

  5. MAPK Plasmids

    Type
    Collection
    ...protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/threonine-... an integration point for multiple biochemical signals. In collaboration with numerous research labs from...

  6. Fluorescent Protein Guide

    Type
    Collection
    ...light to detect, measure, and control molecular signals, cells, or groups of cells with either actuators...genetically engineered cellular receptors that affect signalling pathways within cells. Subcellular Localization...

  7. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... imaging have emission near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin ...hemoglobin and cause undesirable background signals. Near-infrared fluorescent proteins for in vivo imaging are...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...

  9. Promega Plasmid Collection

    Type
    Collection
    ... assays are ideal for real-time monitoring of signaling pathways, investigating RNA regulation, tracking...active enzyme and generate a bright luminescent signal in the presence of a substrate. NanoBiT PPI assays...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...improves protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds...Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through the...

  11. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single pulse...pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten pulses...

  12. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...exactly what iRFPs achieved. Those proteins allowed a signal-to-background ratio in mammalian tissues 20-fold... In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher resolution...

  15. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...molecular level. The brain is constantly influenced by signals it receives from the entire body, making it difficult...advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were very few...

  17. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...reporter. Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    ... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

  19. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...
Showing: 101 - 120 of 267 results