...and increased tariffs present an unprecedented challenge to the scientific research ecosystem, not just...years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to ...resilience and creativity in the face of funding challenges. By strengthening our commitment to collaboration...

...only been available for a little over a year and a half, a small sampling of the research it has already...another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...

...after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, ...and later C. elegans) for the first time. After Chalfie’s work, scientists really started to take notice...Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This great honor...

... some challenges during my internship, but nothing could have prepared me that those challenges would ...exploring my different interests but also a way of challenging what I am used to. Brandon Solis, marketing...

...possible before,” said the Broad Institute's Ophir Shalem. “Whole regions of the genome which were not accessible...depends on your experiment and method of selection. Shalem and Sanjana say they kept a high representation... CRISPR-Cas9 Knockout Screening in Human Cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson...

...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across ... help other teams successfully overcome these challenges, too. Schedule regular meetings Scheduling a...

...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

...team called Running for Rare Disease that runs on behalf of the National Organization for Rare Disorders...HOPE; a fitting superpower for those running on behalf of rare disease sufferers like AJ. As the Blue ... First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy suffering from FOP,...

...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs ...tools for studying translation in living cells. Halstead, et al. Science. 2015 Mar 20;347(6228):1367-671...genes involved in neurogenesis, which had proven challenging using the existing dCas9-VP64 activator. The ...Designing customized fluorescent reporters can be challenging enough, but incorporating multiple reporters ...tools for all-optical electrophysiology A major challenge in the field of all-optical electrophysiology ...destabilizing sequences were added to shorten the half-life of both the luciferase mRNA (3’UTR AUUUA [5...

...translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary ...translation is known to be important in development, and Halstead et al. used TRICK to examine translation of Drosophila...Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An RNA biosensor...

...two genes by one Gal4 driver will produce roughly half as much protein as overexpression of each gene individually...384–391 (2004). PubMed PMID: 15262411. 4. T. D. Southall, D. A. Elliott, A. H. Brand, The GAL4 System :...Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections...

...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

...about 65,000 messages and dealing with email can be half of my day sometimes.” He points out that the copious...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

...longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells poses challenges such as the inhibition... brings together the split botulinum neurotoxin halves to enable VAMP2 cleavage, and suppresses neuronal...

...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases...

...movement is that it provides interesting and unique challenges for intellectual property lawyers. While there...be, an interesting legal and professional challenge.Paratore: The promise there is the ability share the...St. Elizabeth’s Hospital and was associated with Hale & Dorr and Crowell & Morning. Karen was a founding...

...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...talent pipeline. Turnover at Addgene is about half of what is standard in most sectors. The Addgenies...

...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...pTALYM3, pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series ...degredation is the speed at which it works--typical half lives of AID-tagged proteins after the introduction...studying essential proteins. Until recently, it was challenging to apply the AID-tagging system to essential ...expressing and purifying human kinases remains challenging. Small molecule kinase inhibitors usually target...

...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...