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We narrowed to 318 results for: idua

Showing: 101 - 120 of 318 results

  1. MXS Chaining

    Type
    Blog Post
    ...label subcellular structures in live cells. Four individual expression constructs were created, each containing...cloning. The modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...

  4. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...run the journal club. Some are led by a single individual who walks through the paper figure by figure....implications rather than diving deep into the individual data points. All formats should be an open forum...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...successfully expressed as fusions that retain their individual activities (1). Researchers also discovered the...transcriptional activation (TA) domains retain their individual activities when separated (2), and that DBD and...

  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...enables users to conveniently select and deposit individual documents or more complex datasets into the desired... edit to particular groups and/or particular individuals, the issue of third-party access to the ELN arises...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and... amplification, this step also depends on the individual library. So, on each library’s page, you can ...

  10. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...your modified pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids...being explored and it allowed control over each individual component of the system. However, it is easier...

  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...needed specific tools to interpret the roles of individual integrins. The Ha lab — in collaboration with...investigators inhibited other RGD-binding integrins, individually and in combination, showing that among the three...

  13. Evolution of Lab Techniques

    Type
    Blog Post
    ...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... function. Without functioning dystrophin, an individual experiences progressive muscle wasting leading..., but at least partially functional protein. Individuals with these mutations are often asymptomatic, ...

  16. Plan Your Experiment

    Type
    Collection
    ...DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and next-generation...

  18. A Control for All Seasons

    Type
    Blog Post
    ... are likely just natural differences between individuals. Recommended controls for antibody-based experiments...include a control wherein each primary antibody is individually omitted from the staining but all secondary ...

  20. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...different locations and can be cut out of the gel individually. There are a variety of ways to visualize the...your ligation has worked, you will need to pick individual bacterial colonies and check them for successful...
Showing: 101 - 120 of 318 results