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...viral cocktail are injected, by chance lots of individual cells will be transduced with multiple viral ...notice in figure 4b how difficult it is to trace individual cell’s axons, particularly the two green neurons...

...label subcellular structures in live cells. Four individual expression constructs were created, each containing...cloning. The modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple...

...successfully expressed as fusions that retain their individual activities (1). Researchers also discovered the...transcriptional activation (TA) domains retain their individual activities when separated (2), and that DBD and...

...the persistence of residual viral material [6]. The complete removal of the residual viral particles and...

...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and... amplification, this step also depends on the individual library. So, on each library’s page, you can ...

...enables users to conveniently select and deposit individual documents or more complex datasets into the desired... edit to particular groups and/or particular individuals, the issue of third-party access to the ELN arises...

...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ...

...your modified pCAG-EGXXFP plasmid along with the individual gRNAs in their PX330 plasmids (or similar plasmids...being explored and it allowed control over each individual component of the system. However, it is easier...

...needed specific tools to interpret the roles of individual integrins. The Ha lab — in collaboration with...investigators inhibited other RGD-binding integrins, individually and in combination, showing that among the three...

...a single mutagenic primer pair to generate an individual mutant, this technique uses mutagenic primers...The PCR product is digested with DpnI to remove residual starting template. After transformation and harvest...

...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...

... function. Without functioning dystrophin, an individual experiences progressive muscle wasting leading..., but at least partially functional protein. Individuals with these mutations are often asymptomatic, ...

...single-cell labeling is necessary to distinguish individual cells. The Brainbow-1.0 construct contains three... with the vast array of colors needed to mark individual neurons, the system also suffered from a number...

...unpublished data. There are blogs - both hosted by individuals and by organizations like Addgene (a good example...positive result. Beyond these more formal systems, individual labs can certainly institute better experimental...

... are likely just natural differences between individuals. Recommended controls for antibody-based experiments...include a control wherein each primary antibody is individually omitted from the staining but all secondary ...

...sequence the register. In our paper (1), we allowed individual bacterial cells to form colonies on a plate and...annealing temperature, and ~50% GC content) so that individual parts and/or strings of parts may be PCR-ed out...

...different locations and can be cut out of the gel individually. There are a variety of ways to visualize the...your ligation has worked, you will need to pick individual bacterial colonies and check them for successful...

...multiple inserts, enter details for each insert individually.   Figure 9: Add cloning information for the...for your first plasmid and can be used on each individual page (Gene and Insert, Cloning Information, Growth...

...terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose from a growing number...complexes in various hosts. Expression cassettes for individual subunits are first assembled by Golden Gate cloning...

...Insertional mutagenesis, transgene reactivation, residual expression Lentivirus Integrating High Low ...Stable Genome integration Insertional mutagenesis, residual expression piggyBac Excisible Medium Medium...