We narrowed to 161 results for: lentiviral vector

...protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared...

...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert site.... 2018. PubMed PMID: 29300010. miRNA reporter vectors shed new light on mechanisms of miRNA-mediated ...

... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...discuss these in a future post on viral vector parts. Promoter specificity Aside from choosing a promoter based... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

...via a lentiviral vector to cell lines that either stably expressed Cas9 or that had been lentiviral infected...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

...- #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9... 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...

...In addition, several libraries are offered as lentiviral preparations to save you a few steps! To check...Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries Pooled Library ...

...differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101 eBook ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI...

... a) Cells are transfected with three different vectors and flow cytometry is used to sort populations ... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload (...recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors (Janicot ...

... study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, which were ...

....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

...women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these ...

...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag...or search our complete collection and filter by vector type, popularity, and more. Blue/UV Protein Excitation...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...

...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...Gantner, Johannes, et al. “Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular....0197185. Chamness, James C., et al. “An Extensible Vector Toolkit and Parts Library for Advanced Engineering...Rapid Design and Production of Diverse Expression Vectors.” ACS Synthetic Biology, vol. 6, no. 7, American...acssynbio.7b00016. McGee, Abby V., et al. Modular Vector Assembly Enables Rapid Assessment of Emerging CRISPR...

...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...

...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More Antibodies...more. Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

...Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources ...and in vitro methods are similar except that the vectors carrying the transdifferentiation factors are directly... (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234...., Human induced pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928...mouse induced pluripotent stem cells without viral vectors. Science, 2008. 322(5903): p. 949-53. PubMed PMID...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...

...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

...millions of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...cells. Remove this pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing...