...neural connectivity (Nassi et al. 2015). The recombinant viruses typically used in neuroscience research...transmitted transsynaptically). This is because the recombinant forms of these viruses typically used in research...characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. ...Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013;8:1583...

...general availability of their recombinant antibodies. Since recombinant antibodies are a newer technology...chose monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity...ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

...Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] ..., 1984). The strategy they landed on was to recombinantly append their proteins of interest with unrelated...have moved to producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable...

... program is the right fit for you without the financial and logistical stress that accompanies traveling...faculty, students, and each other. Before COVID-19, financial and logistical obstacles could make touring potential...COVID-19? How are students funded? Are there any financial aspects that are confusing? During a traditional...interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage in the...

...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...://doi.org/10.1016/j.cell.2024.01.028. New recombinant antibodies for neurodegeneration research   By...-MAPT [AT8] and Anti-APP [6E10-A5]. Both are recombinant mouse monoclonal antibodies targeting the human...Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results...

...Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular... K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … ...Packaging of genomes can limit production of recombinant AAV-2. Gene Therapy, 6(7), Article 7. https:/... Snyder, R. O. (2010). Characterization of a recombinant adeno-associated virus type 2 Reference Standard...

...lower values than Nanodrop, although most protocols have been designed with Nanodrop as the quantification...lentiviral particles. See below for options if the recombinant band makes up a significant proportion of the...solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each different DNA measurement...burden on the bacteria. Bacteria that contain a recombinant plasmid likely grow faster in the closed system...

...repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR) pathway in mammalian...your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when a ...20): 1-9 (2020). 10.1038/s41589-019-0432-1 Mehdi Banan. Recent advances in CRISPR/Cas9-mediated knock-ins...2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar, C. E., et al. CRISPR-Cpf1...

...-lot. The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...plasmid sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different...non-specific binding; lot-to-lot variability Some recombinant antibodies Not always available; not always... Monoclonal antibodies Increased cost Recombinant antibodies Not always available; cost varies...non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not always available...

...improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 (2015): 1–14. PubMed...Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular Biology of Biotin Attachment ... PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic biotinylation of proteins...

...ligand is attached to a protein binding partner or nanobody that targets the native receptor allowing the ....2021.778900 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L... B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J...science.aaw5202 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L...

...long-read sequencing techniques, such as Oxford Nanopore sequencing or PacBio SMRT sequencing, (which differ...long-read sequencing (1-25 kb), such as Oxford Nanopore sequencing or PacBio SMRT sequencing. These longer...Levene SD. Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis. Methods Mol Biol...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...Roger Y. Tsien. "Specific covalent labeling of recombinant protein molecules inside live cells." Science281.5374...

..., J Biol Chem 2014 Apr 25;289(17):12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. ...of interest and co-expressing its replacement recombinant protein with a FKBP domain and a mitochondrial... FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) and then sequesters the protein...Rinehart lab reagents for improved expression of recombinant phosphoproteins Protein phosphorylation is one... Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck Institute...

...with a relatively small amount of DNA (in the nanograms to micrograms scale depending on the experiment...more importantly, the DNA needs to be free of contaminants, such as bacterial endotoxins, that could affect... Another is the possibility of carrying over contaminants from the plasmid prep process into the final...final plasmid eluate. Possible contaminants can sneak in from almost any step of the process and include ...

... blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog series will help...ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells...’s lab developed a lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins...

...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer...heterotrimeric G protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the...

...conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies are plasmid-based and ...is routinely used to increase the utility of recombinant antibody tools, the resulting chimera may have...

...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...., HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol...

...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...