...Whenever possible, include positive and negative controls. Positive controls typically include cell lines or...several variations of the protein transfer step including wet, semi-dry, and dry but all follow the same... Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot ...data sheet thoroughly. Vendors will frequently include suggestions for working concentrations, blocking...blocking buffers, and incubation times. If the vendor includes publications that cited the antibody, consider...It is important to note, however, that you must include a loading control when assessing protein abundance...western blot. When planning a western, take care to include the proper positive and negative controls, choose...

... allows it to be used in more E. coli strains including the popular BL21(DE3) (Li et al., 2021). This ...original pCas/pTargetF but has many other advantages including shorter plasmid curing time. pCRISPomyces for ...Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces.... INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the...essential genes in a wide variety of bacteria including in pooled, genome-wide, formats. Interested in...proteins involved in the activation of transcription including transcription factors, phage proteins, and RNAP...Adding complexity, they found that they could also include a CRISPRi system for multiplexed modulation. CRISPRa...

...results, conclusions, and acknowledgements sections. Some other additions you might wish to include are a...navigable and efficient; your questions, data, and conclusions should be clearly identified and interpreted ...smaller than in other poster sections, so you can include a lot more. Font size: Making your poster readable...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are inserted. ...the central region, and a scannable barcode is included that links readers to more information if they...background but want to add a bit more “flash,” you can include a subtle gradient (as is shown in all of our illustrations...

...collection of plasmids for coronavirus research, including a handful of plasmids that have been in the repository... plasmids used for this study at Addgene which include a C-terminal deletion in the spike protein to improve...Plasmids deposited from Alejandro Balazs’s lab includes those for generating pseudovirus and those encoding...the widely used qPCR and antigen tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had...for detection have been made openly available including those from Andrea Pauli and Julius Brennecke. ...19 diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase ...untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection...

...data, you can even include your own data in a ReFigure. For example, you might include a confirmatory image...cofounder of ReFigure. Reading and exploration including replications and experiments resulting in “negative...also eLife, PLOS and Figshare). Some use cases include: Cataloging a reagent’s performance as in this...remain hidden from research papers as they are not included in traditional publications. By submitting your... currently supported by ReFigure beta) you can include it in a ReFigure. ReFigures are good for you and...

...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies and...antigen, and performance in various applications are included in the full webpage. Educational Resources...discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence, conjugates...over time. Through our incredible community, including your support, we have enabled scientists to easily...

...many roles that actively require these skills, including: Science Reporter Scientific Sales Epidemiologist...Careers where you’ll actively use these skills include: Science Reporter Scientific Illustrator Research...outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant...etc) are highly desirable in a range of roles, including: Science Reporter Technical Writer Statistician...position. Questions you’ll want to find answers to include: Will you be sitting at a desk all day or be on...to the content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure...

... software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be detected...greater than our previous mapping software and now includes Type IIS restriction enzymes that are commonly...tooltip information and sequence analysis tabs including: Maps This is the first tab you’ll land on...enzymes that cut the given sequence. The table includes enzyme names, the locations where the enzymes ...features detected in the plasmid sequence. The table includes feature names, locations, sizes, colors used to...detected in a given nucleotide sequence. The table includes primer names, sequences, binding site locations...

...things you need to include in your manuscript. What information do I need to include in my paper? More ...and your results more valuable. Some journals, including F1000Research, also ask for the raw data behind...good quality and whether they agree with the conclusions of the article. These initiatives are another... to your submission, briefly summarizing the conclusions of your paper. This will help the editor handling... have ignored their comments, they will not be inclined to approve your paper. Rush your submission or...

...strategies beyond SpCas9, or species not currently included in Green Listed.  Provide the list of genes ...Input” box. Choose from different “Options”, including adding adapter sequences for cloning purposes.... output folder containing several files. These include: Output: A text file containing full information...information about the suggested gRNAs to use, including all information provided in the original reference library...Output_Compact: A text file that describes which genes were included, and how many gRNAs were identified for each gene... gRNA, as well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all...from the software, and my hope is to continue including other libraries. As of now I have focused on human...

...from the company LinkedIn page. This can include news, inclusion, culture and connections. Start your interview... is within a few days after you’ve met. Always include a note about where you met IRL (in real life) and... a connection request to any of these people, including and especially the hiring manager. This is considered.... Follow all the rules of good resume writing. Include examples of concrete accomplishments in both the...the technical and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot...

...results that ultimately builds up to an overall conclusion(s). In some articles, the data are presented ...to build a case for a certain model or overall conclusion. In others, the experiments are geared towards...possibilities until the results focus in on a particular conclusion. Many papers aren’t always written this way and...the results to see if it leads you to the same conclusion. Personally, I like papers that can end with ...are appreciated) to visually sum up all of the conclusions. In any case, the reader should give a critical... the relation to how they are supporting the conclusions. Look at the figures and write down your own ...

...genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic or .... We do this through a variety of activities, including creating educational materials and lesson plans...to hamper efforts to engage these communities, including those by researchers hoping to increase community...leadership and communication experience. These included taking extra coursework and attending seminars...of organizations in public engagement efforts, including giving public lectures, writing science articles...our experiences in a variety of other settings, including “conventional” academic venues such as scientific...

...experimental processes, results, and subsequent conclusions. Being vigilant about these errors is an important...easy to share. However, the cloning process — including the propagation of plasmids in bacteria — has ...and money on optimizing experiments, to false conclusions.  Figure 1. Potential points where DNA..., indicated by exclamation marks. Error points include places where DNA is being created, such as PCR ...believe may affect the function of the plasmid. This includes point mutations, frame shifts, missing elements...require additional attention during analysis. These include topics such as: locations in a plasmid that are...

..., and include information on tested antibodies against the target. Helpfully, this also includes clone...antibody for flow cytometry experiments. This will include a discussion of the Human Leucocyte Differentiation...approach Genetic knockout-based validation can conclusively prove the ability of an antibody to detect the...separation and reduce any background staining. This can include using: different blocking reagents; titration of...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown is...molecules expressed on human blood leukocytes, including antibodies against these markers. The 371 CD markers...

...recently, online journal clubs have become popular, including those based on Twitter. At my former academic ...recurrently carved out. This notice typically should include the paper, any supplemental data, software repositories...feedback could be solicited via multiple avenues including casual request via email, in person, or instant...group and format but should almost universally include the questions “Would you attend this journal club...you can point to. Does the feedback you receive include the sense that people learned something new? Did...continually evaluated and assessed for relevance.  Concluding thoughts Journal clubs are a great way for scientists...

... control for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and...off-target cutting of only a handful of guides. It’s unclear if off-target cuts confound the results of high-throughput...without a reduction of on-target activity, but it’s unclear if this holds true in genome-wide screens.   ...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

... FP fusions including erroneous quantitation of fusion protein levels. It remains unclear whether the ...chemically distinct subcellular environments, including the organelles that compose the secretory pathway... significant post-translational modifications including glycosylation, disulfide bond formation, and proteolytic...imaging in a variety of cellular compartments including the secretory pathway (Figure 2), the inner membrane... Anatomy and Structural Biology. His interests include the quality control of secretory proteins in the...

...experiment lacking the proper controls are invariably inconclusive and unreliable. Proper controls provide the ...human cells. From this result one could simply conclude that the shRNA didn’t work, as the expression ...control plasmids Part of planning your experiment includes determining what factors need to be controlled...microscopy images resulting from our experiment that includes the aforementioned internal control and is consistent...to note that optimal experimental conditions, including how much plasmid DNA to use for any individual...plasmid-based experiment, untreated cells should be included as these provide the baseline/standard against...

... open access publisher has required authors to include a note as to where readers may locate data supporting...information) for assessment of veracity of the conclusions drawn. The main difference with the PLOS policy...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...information is made available to everyone at once, including the generators. This practice doesn’t appear to...The fact that so few authors now query what to include suggests pragmatic adoption (although it might ...access to data is important, what data should be included? We should recognize that storing data in a sharable...