We narrowed to 308 results for: pET

...during transformation First, use enough DNA and competent bacteria to ensure all components of the library...library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications...

...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene...

...of armor to protect their host from predation (Petroni et al 2000). With a better understanding of how... Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & ...

...guide for the quest of a perfect "fit". How competitive is the lab? Some labs have only one author plus...labs around the world. You may prefer a more competitive style to spur you to succeed or you might enjoy...

...help manage details. For example, setting up your pipette tips to match your plate layout can help you track...too - one of my coworkers would build a wall of pipette tip boxes when she particularly needed to focus...

...-mutagenesis also requires making your own electrocompetent cells in which the phage recombination machinery...Purified Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...

...(anthocyanins), and the plant circadian clock. Petunias have been a model organism for plant research ...mechanisms and components of the circadian rhythm in petunias. Because of the extensive research that has come...

...and the efficacy of your competent cells (when they get old, they lose competency). If all the above is ...

...particularly enriched in highly transcribed regions and repetitive regions, such as telomeres and centromeres. These...presumably due to their abundance of R-loops and repetitive sequence. All of the R-loops described above...

... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

... formation of replication competent lentivirus (RCL). Replication competence can arise from recombination...

...into the host genome, the virus is replication incompetent, meaning it can’t replicate and continue the ...Viral cargo-specific safety   After replication competency, the second concern with virus safety is the ...

... a team at UVic to compete in iGem, an MIT-sponsored synthetic biology competition. In 2010, he founded...

...70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather decant ...material that is intended for reuse (e.g. Pasteur pipettes for liquid waste) should be sterilized before ...

...CRISPR-transposons by the Sternberg and Zhang labs (Peters et al., 2017; Klompe et al., 2019; Strecker et ...225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment...

...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

...division. These non-plasmid-containing bacteria can compete with and confound the goal of your experiment —...express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy...

...virions) are not expressed. Note that replication-competent AdV vectors also exist and are used in certain...topic, which is that natural AdVs, replication-competent viruses with a recombinant genome, and adenoviral...

...system, the possibility of creating a replication-competent virus via recombination between the transfer and...recombination events necessary to create a replication-competent virus. Second, Tat is eliminated through the addition...

...single colony with a sterile flat toothpick or pipette tip and swirl in a small amount of sterile water...loading dye with glycerol to your samples before pipetting them onto the gel. The figure above summarizes...