We narrowed to 166 results for: pcr

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Neil McKenna and Bert O’Malley of the Nuclear Receptor Signaling Atlas (NURSA) provide an introduction to nuclear receptors and more.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Learn how to produce, purify, and titer adenoviral vectors for your research.

Quickly isolate mammalian cells clones using the agarose cloning method.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

Ready-to-use retrograde AAV available from Addgene's viral service. Retrograde construct from Alla Karpova at Janelia Research Institute.

Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's lab at Caltech.

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

Learn about the differences in virus production between insect cells and mammalian cells.