Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 210 results for: pcr

Showing: 101 - 120 of 210 results

  2. Working with Nuclear Receptors

    Type
    Blog Post
    ...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...

  3. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers ...Tips on designing primers for PCR Protocols...primers are necessary when running a PCR reaction. One needs to design primers that are complementary to the...protocol video below to learn how to design primers for PCR. Reference Page Top Index...

  4. Ligation Independent Cloning

    Type
    Protocol
    ...sterile dH20 to 40 μl Step 4: Amplify Insert by PCR Perform PCR amplification of your insert following the ...ensure a melting temperature between 50-60°C for your PCR primers. Step 2: Linearize Vector In this example...like this: Note: Elute your purified digestion and PCR products with sterile water (instead of TE buffer...important to remove all free nucleotides from your PCR product before proceeding, as they will interfere...Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in the presence of dCTP (not ...

  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...reaction. This will allow you to quantify the amount of PCR product that comes from amplification of the original... titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral prep...first converted to cDNA and then quantified using qPCR primers targeting specific viral components such...assays depending on the vector backbone. When using qPCR based methods for viral titration consider using...most accurate method of lentiviral titration uses qPCR to measure the number of proviral copies that have...cell line known to contain 1 integrated copy of the qPCR target gene. Developing the proper control line ...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...of a downstream application such as quantitative PCR to assess gene expression. There are several things...

  9. A Look at Addgene's QC Process

    Type
    Blog Post
    ...include places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender.com...

  16. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Watch the Video! Polymerase Chain Reaction (PCR) Basic PCR protocol with tips and FAQ Watch the Video!... digest and ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning...Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer ...preparation Watch the Video! AAV Titration by ddPCR Use ddPCR to measure the physical titer of your adeno-associated... virus preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus...

  20. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Ehrhardt, A., & Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated...aliquots of virus. AAV titration by qPCR Overview: AAV titering by qPCR quantifies the number of genome-containing... hours for the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more...remember to make one or two small aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws... included. While the physical titer generated by qPCR is useful, I test a few different concentrations...
Showing: 101 - 120 of 210 results