We narrowed to 110 results for: quo;
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TypeProtocol...well and sterilize through a 0.2 µm PES filter. Aliquot and freeze upright at -20 °C Procedure Section ...Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir to check...
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AAV ddPCR Titration
TypeProtocol...contaminating reagents we recommend making small aliquots of master mixes, primers, and probes prior to ...gently uncap the tubes. Add 5 µL of the previously aliquoted 1X dilution buffer from step 9 to the NTC tube... -
Kit Free RNA Extraction
TypeProtocol...your entire RNA sample, consider making smaller aliquots of it and storing those in -80°C. Option #2 - ...your entire RNA sample, consider making smaller aliquots of it and storing those in -80°C. Solution D Protocol... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...recommends that you use the virus immediately or aliquot the media into smaller tubes to prevent multiple...Sterilize by passing through a 0.22 μm filter. Store aliquots at -20°C. I.3. Warranty Information Addgene is... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommended... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...idea to take a fresh tube, thaw it one time and aliquot individual tubes of 5, 10 or 20μL for storage at... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...possible. Store at 4 °C for short term (2 weeks), or aliquot and store at -80 °C for long term. Sample Data ... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol...culture cannot fit into a single centrifuge tube, aliquot it into several tubes/bottles. Remove the supernatant... -
AAV Titration by qPCR Using SYBR Green Technology
TypeProtocol...validated standard curve is obtained, make a small aliquot of each standard (enough for 1 or 2 plates) and... -
Lentivirus ddPCR Titration
TypeProtocol...contaminating reagents we recommend making small aliquots of master mixes, and primers/probe mixes prior...