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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 799 results for: tec

Showing: 101 - 120 of 799 results

  1. Brain Initiative Collection

    Type
    Collection
    ...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...

  2. Weighing Reagents Protocol

    Type
    Protocol
    ...for a stock mixture is an essential laboratory technique, as imprecise measurements can affect the outcome...weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials...

  3. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...insert and vector backbone are known and thus this technique can be quickly used to verify your plasmid. The... should be. A useful restriction enzyme based technique for verifying plasmids like this is "plasmid fingerprinting...

  4. Centrifugation

    Type
    Protocol
    ...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...higher speeds and are used for more specialized techniques. Regardless of the type of centrifuge you use...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ...ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from the ssDNA-specific endonuclease... is to “stitch” together several oligos. This technique is especially useful for introducing promoters...

  6. Using a Light Microscope Protocol

    Type
    Protocol
    ...advantage of the physical properties of light to detect small objects. Two of the most important properties...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

  7. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...the literature for an appropriate sterilization technique if you are working with any weird and wonderful... into your ~60 ℃ molten gel mix using sterile technique. Swirl the agar bottle to ensure even distribution...

  9. Cre-lox system

    Type
    Collection
    ... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...preparing and handling lentiviral particles. Personal protective clothing should be worn at all times. Use plastic...gene delivery in vivo. Zufferey R et. al. Nat Biotechnol . 1997. 15(9):871-5. PubMed . Self-inactivating...

  11. Protocol - How to Streak a Plate

    Type
    Protocol
    ...do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start by streaking...

  17. AAV Production in HEK293 Cells

    Type
    Protocol
    ...meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (ideally...

  18. CRISPR Library Amplification

    Type
    Protocol
    ...to lysis by freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the...

  19. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that occur...primary methods of off-target detection: biased and unbiased. Biased techniques only sequence certain sites...: Biased Genotyping Options Technique Sequence Information Detection Limit Advantages Disadvantages...highly sensitive with detection levels as low as .01% (Hendel et al. 2015). Low detection rates mean the investigator...off-target mutations if they remain undetected using these techniques. Frequencies of off-target mutations...have a minimum detection limit of 0.1% (Tsai et al. 2015). This contrasts with detection frequencies of... Table 2: Unbiased Genotyping Options Technique Detection Limit Application Advantages Disadvantages...

  20. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... for detection RANbody is detected enzymatically with HRP substrate More sensitive than detecting antigen...require a secondary antibody for detection. Instead they are detected with tyramide signal amplification...and is useful for detecting GFP. Spaghetti Monster tagged RANbodies can be detected either with a labeled...sensitive than HRP RANbody detection, it is approximately as sensitive as detecting the same antigen with ...RANbodies are easily detected with a labeled anti-chicken antibody. Chicken IgY RANbody detection is less sensitive...sensitive than HRP RANbody detection but approximately as sensitive as antigen detection with a standard primary... When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen...
Showing: 101 - 120 of 799 results