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We narrowed to 1,073 results for: TOR;

Showing: 591 - 600 of 1073 results
  1. The Pleiades Promoter Project

    Type
    Collection
    ...Project - A regulatory toolbox of MiniPromoters to drive selective expression in the brain.... Depositor Collections...., Wasserman, W. W., Simpson, E. M. (2010). A regulatory toolbox of MiniPromoters to drive selective expression...
  2. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the new search...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...you come to Addgene to find the plasmid or viral vector for your next experiment, that you can find what...
  3. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation...current postdocs will be academic principal investigators. She also pointed out promising new practices...increasing the value and priority put on good mentoring and applauded the new requirement for PIs to include...opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments were about ...
  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor... Baird said. “They are all designed in the same vector backbone with very similar restriction enzymes....Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...shutter its doors. “We wanted to give all of these vectors to the scientific community and contribute in a...community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson...
  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...considerations, such as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA... Although downstream work is required, several vectors can be found at Addgene that could serve as an ...an excellent start (#22988, #137884). Both vectors allow the creation of amiRNAs based upon miRNA precursors...method. Instead of using a fluorescent output to monitor RNAi, we engineered a positive selection reporter...
  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...chance vs. the factor that is being experimentally manipulated. Below are several core factors of statistics...variables – factors which are not affected by other variables (cause). Dependent variables – factors which...total crop production and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...
  7. RUBY-Red Siliques

    Type
    Blog Post
    ...Willoughby.   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al...., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors ...
  8. What the HEK?

    Type
    Blog Post
    ...model for cancer-specific research. Membrane receptors and ion channels are often exogenously expressed... degrade over time in culture. This can affect factors that could skew research results (gene expression... Especially if your research interests include receptor biology, ion channels, or involve human protein...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional resources...
  9. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal...
  10. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...different story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start...spent your life making connections and finding mentors -- well, it's time you call upon these people for... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...
Showing: 591 - 600 of 1073 results