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We narrowed to 819 results for: cell

Showing: 591 - 600 of 819 results

  1. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ... can be used to deliver fluorescent proteins to cells as determined by the serotype. You can use these...determine if your AAVs are getting to the right cells using your particular delivery technique and conditions...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...

  3. CRISPR References and Information

    Type
    Collection
    ...interest and correspond to edited and non-edited cell populations. For more information, see the Hsiau...138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook...Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...experiments possible in a wide range of organisms and cell lines. In C. elegans, one of the model organisms... efficiency. Finally, as shown in Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous... community. Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression...homologous recombination donors in cultured Drosophila cells. Nucleic Acids Research 42:e89–e89 . https://doi.org...

  5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9 or that ...almost all genes involved in the production of a cell surface glycan that’s required for ricin uptake....reveals pathways underlying ricin susceptibility." Cell 152.4 (2013): 909-922. Pubmed PMID: 23394947. Pubmed...

  6. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...optogenetics, ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321...Dynamics of retrieval strategies for remote memories. Cell. 2011;147(3):678-89. Pubmed PMID: 22179551. Pubmed... remote contextual memories. This study is an excellent example in which the authors started with a brain... research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...

  7. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ...components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics Background...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    .... This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...engineering project at BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge... in an Open Source Lab: from CRISPR to Cats Stem Cell Models for Disease & Open Science: Interview with...
Showing: 591 - 600 of 819 results