We narrowed to 1,073 results for: TOR;

...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of... have low knock-in efficiency. Together, these factors prevent stem cell researchers from generating clonal...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this was possible...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...success of knock-in experiments depends on many factors. Some of these include: Most importantly, the...

...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...It’s also important to determine if the human interactors we found in our map have an impact on virus replication... replication. While we and our collaborators are starting these experiments, there's so much to do that...

...elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug, including...these algorithms to model individually-specific factors, such as previous exposures to that specific antigen...assessments, as well as analyze the contributing factors that in silico prediction algorithms fail to capture...10.1634/theoncologist.2016-0061WHO. International monitoring of adverse reactions to drugs: adverse reaction...WHO Collaborating Centre for International Drug Monitoring, Uppsala, Sweden, 1992. Mattei, A. E., Gutierrez...

...researchers, it can expose them to a new institute and mentor that they may want to consider for future work ...professor or institute, ask if you will have a bench mentor or anyone else available to directly help you during... during your visit. Reach out to your bench mentor (if you have one) to communicate your plan as well.... comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read...

...looping factors developed thus far for these latter experiments, such as transcription factors tethered...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation of ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser...experiments and grant writing. You have to enjoy the mentoring role.” She points out that new PIs may not recognize...student or postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...

... are three main pathogen clearing functions or effector functions of the constant regions of antibodies...functions: Recruitment of immune cells that express receptors that recognize the Fc portion of different antibody...domains, or Fc region, can be bound by special receptors that transport antibodies through cells and into...role in allergies. They are found bound to IgE receptors on the surface of basophils and mast cells, two...of an immune response since it also changes the effector function of the antibody. Isotype switching is...

...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989...be individually optimized for each of the three factors: the ncAA, the tRNA, and the tRNA synthetases. ...balance can be extremely tricky to find: each of the factors can negatively impact both translation and fidelity...complicated.” And Ryan Mehl would know. He’s the director of GCE4All, an NIH-funded center located at Oregon... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...professional network such as co-workers, professors, and mentors. Ask for specific guidance on grammatical errors...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...practice a set of common questions with a peer or mentor. Several days prior to the interview, inspect your...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...