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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 819 results for: cell

Showing: 601 - 610 of 819 results

  1. KLF Research Plasmids

    Type
    Collection
    ...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...

  2. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... that converts sucrose into a toxic metabolite. Cells that have undergone a double crossover will not ...contain sacB, allowing them to grow on sucrose. Cells that have only gone through the single cross over... from Harvard. Jake received his MD and a PhD in Cell and Molecular Biology from the University of Pennsylvania...Giacalone et al., 2006). The Chen lab demonstrated excellent counter-selection which enabled very efficient...desired deletion directly into cut vector with excellent efficiency using a commercial isothermal assembly...

  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...translation of many stop codons and detrimental effects on cell growth. The C321.ΔA strain was genomically recoded...is particularly interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion..."The origins of protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID: 11988757...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  6. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...

  7. TALEN Expression Vectors

    Type
    Collection
    ...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...III CRISPR-Cas Immunity. Cell, 161(5), 1164–1174. https://doi.org/10.1016/j.cell.2015.04.027 Stella, G.,...and is able to target and edit RNA in mammalian cells. Type IV Type IV is the middle child (like me!) ...-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://doi.org/10.1016/j.molcel...biotechnology toolbox for and beyond genome editing. Cell & Bioscience, 8(1). https://doi.org/10.1186/s13578...

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...
Showing: 601 - 610 of 819 results