We narrowed to 819 results for: cell

1. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

2. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

3. Lucky Thirteen: Guest Contributors of 2023

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   Blog Post

   ...ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   ...Different AAV serotypes have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have...most cases with VSV-G that generally mediates broad cell tropism and has a high percentage of neuronal targeting...

5. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ... channels in living cells. Multiple properties of a channelrhodopsin, such as cell localization, kinetics...

7. Handling Plasmids from Addgene - Purifying Plasmid DNA

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   Protocol

   ...min at 12,000 g. Notes: Pellet contains proteins, cell fragments, salt and other extra particles from solutions...

8. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...origin of cellular life. His lab has long hypothesized that life started with RNA and a simple cell membrane... circular RNA they have identified resulted in a cell migration defect. They have created a circRNA website...

10. The Materials Science of Optogenetics Experiments

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    Blog Post

    ...Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674. doi: 10.1016/j.cell.2015.06.058. Pubmed PMID: 26189679...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29...Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...