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Showing: 621 - 630 of 971 results
  1. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...affected the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't... prominent role in the scientific endeavor – particularly in fields like stem cell biology where questions...about his clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work...
  2. Management for Scientists: Delegating is Key

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    Blog Post
    ... the heart of delegating for success.  Meet regularly before during and after the assignment to discuss...review project progress. I recommend putting a regular check-in meeting on the calendar even if it is ... Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here...
  3. Resume Writing for Non-academic Science Careers

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    Blog Post
    ... data, then the chance is that you have handled large data sets and are familiar with statistical analysis...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., many universities make their job postings and salary expectations public, so start by looking at these...
  4. Advice for Scientists Starting a Lab

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    Blog Post
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation... time in his office, he gradually incorporated regular group meetings, journal clubs, and focused sub-...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...
  5. Finding and Joining Your Dream Lab

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    Blog Post
    ...engagement and access to growth opportunities, not salary and benefits. Therefore, lab selection should focus...Alternatively, perhaps the lab publishes and presents regularly with labs around the world. You may prefer a more... making this choice.  How big is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging...
  6. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material...virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (293E or 293T cells), allow for episomal.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...
  7. Antibodies 101: Flow Cytometry Controls

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    Blog Post
    ...unsure what your population will look like for a particular marker, you can use the reference plots provided...isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined...and then add in your isotype control for that particular marker. This enables you to identify any non-...
  8. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...Fig. 1B). Tracers are typically delivered to a particular location and they either trace downstream from..., you can map what regions are signaling to a particular site while also activating or inhibiting gene...these vectors can exhibit spurious expression, particularly at the injection site (Fischer, KB. 2019). The...
  9. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...used to investigate the effects of activating particular signaling pathways in specific cell types (typically...typically neurons). While all of these viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double...animals, where cre expression is restricted to a particular cell type. The human synapsin promoter further...
  10. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... almost all of the attendees were going to be molecular biologists. This was not at all the case. The ...neurobiology (and really in all of biology) require large data sets. Whether these data sets include videos... make the virus for you. Neuroscientists are particularly interested in using AAVs because they provide...
Showing: 621 - 630 of 971 results