We narrowed to 1,073 results for: TOR;

... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates... coveted Blue Flame is more than an award for depositors and inspiration for our updated mascot, it’s ...model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you planning?...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

...acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength in the... forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires...that the protein could be taken directly from A. Victoria and expressed in any organism while still maintaining...promoter of interest can be used to effectively monitor gene expression from that promoter in a given cell...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection...

...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...always get done, but you’ll have time to read a story to your baby or run that extra two miles which is...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

...much information as possible from depositing laboratories and associated publications, but our quality...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...user interface, so our QC team could initiate and monitor the entire pipeline. Benefits of Nextflow for ...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with NGS ...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...requiring either expressing it with additional cofactors or using significantly truncated forms that limit...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...abscessus, an increasingly common human pathogen notorious for its high resistance to antibiotics. Several...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

...genetically encoded fluorescent probe. Several factors contribute to the popularity of GFP including (...organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter ..., proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains that...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein binds...Smaller genetically encoded probes are based on cofactor binding proteins as described above. The smallest...

...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record...means to manage their work, combining data, team, inventory and project management functionalities with experiment... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

...motifs within transmembrane proteins, such as receptors, or post-translational modifications on those ...) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells and...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note that when.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...equipment, Fluorescent emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...