Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars;

Showing: 631 - 640 of 971 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    ...for either tissues or cells. The process is very similar for both, though tissue samples do require an extra...thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s all the fuss...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...noninvasive methods for manipulating cell activity, particularly in the brain, and holds promise in the development...development of future therapies. Our popular plasmid encoding a Cre dependent version of the DREADD hM3D ...on availability date or to be notified when a particular catalog item is available for order please email...

  3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...cell type? You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

  5. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...and not just the subset of those that return a particular result. Anything else that occurs during the ...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...Promotion Guidelines; preregistration to increase clarity in study design and analysis; removing publication...

  6. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to... (HypaCas9). This nuclease showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9...degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent alternatives...

  7. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...could code the best operations software possible. Similarly, when the developers needed to know information...relationship-building and influence. You’ll need a large deal of influence and strong relationship-building...Technologies for the North America region. She is particularly interested in DNA sequencing tech, and the intersection...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    ...offset due to the nanobody-GFP interaction). The modularity of the system provides researchers better flexibility...comparable to commonly used CRISPR/Cas9 methods for large inserts. However, this system has a much lower error... a fluorescent protein coupled to a specific subcellular localization peptide. By combining three different...

  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

  10. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...
Showing: 631 - 640 of 971 results