AAV Production in HEK293 Cells
Type
Protocol
...After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution...
We narrowed to 938 results for: Kin
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What's New in CRISPR - Spring 2019
TypeBlog Post...evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used for A•T to G...Valcyrie is a CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis ... -
Let There Be LITE Plasmids
TypeBlog Post...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post... Environmental Protection Agency, which started making regulations such as preventing manufacturers from...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding...addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made an appearance there too) and... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...Balance - Vini describes how she balances working in the lab and working with SITN. She points to collaboration... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You can ... filter the table and find exactly what you're looking for. Search using the box highlighted in red above... -
Science as Art
TypeBlog Post...for all to enjoy. The largest competition of this kind is Princeton University’s Annual Art in Science ...Time magazine . Other smaller competitions are now taking place and gathering momentum, including in Cambridge... -
What's New in CRISPR - March 2020
TypeBlog Post...fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells that express both the dCas9 construct and... by co-delivering TET1 and protein factors for linking methylcytosine oxidation to DNA repair, which increases... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...