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We narrowed to 997 results for: lars;

Showing: 641 - 650 of 997 results
  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...
  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach Multiple...promoter-FP fusion for the gene of interest. L1/L2 larvae from these strains are then heat shocked to remove... this system Dickinson et al. have developed a modular system that requires very little hands-on labor...
  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ... smaller than the vector) a 3 insert : 1 vector molar ratio will work just fine. We recommend around 100ng...proportionally. 1μL of ligase should be sufficient for larger ligation reactions. Because ligase buffer contains...vector Cut vector + Background due to vector re-circularization - most useful for phosphatase treated vector...vectors, this ratio is really meant to refer to the molarity of DNA ends available for ligation. Simply put...
  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...problem can be attributed to communication, and in particular communication about experimental methods and ... and funding, so institutions and funders, to a large extent, hold the keys to getting transparency and...journals including Trends in Cell Biology, Cell, Molecular Cell, Developmental Cell, and Cell Stem Cell.  ...
  5. Celebrate National Book Month with Addgene

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    Blog Post
    ...insects, mammals, and plants, among others. I particularly enjoyed the emphasis that microbes are neither... "The Mistborn series is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000...Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling...
  6. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...being used to study SARS-CoV-2. The resurgence in popularity of the hACE2 transgenic mouse strain, for example...murine ACE2 (Subbarao et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV...SARS-CoV-2, this mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution...
  7. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking if we were teaching classes...provide all our members the opportunity to do molecular biology, we’ve developed community projects. Recently...protocols so that anyone can engineer yeast to do similar things. The focus is to make yeast engineering ...
  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Regulating translation is key to cellular function, especially during development or stress. With ribosome...explore the complexity of translational control in cellular physiology. Ribosome profiling Ribosome profiling...all of the mRNA bound-ribosomes in a cell. When cellular mRNAs are digested with ribonucleases, ribosomes...
  9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...FLiPs in a variety of research applications and particularly notes their ability to be precisely spatio-temporally...hopes that other researchers will be able to use similar designs to create a wide variety of enzymes and...proposes using this sensor to detect HMOs found on cellular surfaces. After incubating the sensor with bacteria...
Showing: 641 - 650 of 997 results