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We narrowed to 971 results for: lars;

Showing: 641 - 650 of 971 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ... you will be screening a large number of colonies, we recommend using larger plates. Many labs use 100... plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...good idea of what that volume looks like in your particular plate. For the remainder of the plates, pour ...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get... get PCR amplification. We can start similarly, taking the final 18bases of the ORF, including the stop...will need to use a phosphatase to prevent re-circularization of the vector. You should treat your digested...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ...

  3. Western Blot

    Type
    Protocol
    ...high molecular weight proteins and higher percentage acrylamide when immunoblotting low molecular weight...and reagents from other vendors should produce similar outcomes when using these protocols. However, please... ideal lysis buffer will vary depending on the cellular location of the protein of interest. RIPA buffer...buffer will vary depending on the sample type and cellular location of the protein of interest. You may need...

  4. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...worry about getting support from depositors. We regularly hear how much they see the value in depositing...) and even on 60 Minutes. We are blessed with a large community of scientists who support us by depositing... I checked we were ranked as the 21,806th most popular website in the world. I know, right? Upgrades to...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Check out our molecular biology protocols Find protocol videos on our website Read our Molecular Biology Guide...many samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An ...

  6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...or other synthetic biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise it would be ...achieve!) Each field has its own framework: a particular way of organizing and connecting information ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...

  8. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...Support Team here at Addgene, so troubleshooting molecular biology experiments was in the job description...fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving...result of resolving the issue. Depending on the particular problem, you might even be expected to write ...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    ... repositories like Addgene provides large-scale savings. How large? Using Doench’s calculations and the...purchases can raise the cost anywhere from a few dollars to over a hundred. Of course, these were all 2018...

  10. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...microscope showed us that, in monkey neurons, the large majority of the gold particles were in the cytoplasm...after it has been administered. Although we found a larger proportion on the membrane in mice neurons, most...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...
Showing: 641 - 650 of 971 results