...correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...

...contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 ...which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes Cas9 extremely... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

...Excellent scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...careers in science communication The strength of story telling Science communication: 9 strategies to get...

...of emitted photons before they are destroyed. Factors that affect photostability The photobleaching rate...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation... for each of these conditions. On top of these factors, environmental conditions such as cellular redox...

...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...RNA-binding protein that’s highly expressed in neural progenitor cells. Knock-down of QKI decreased Zika viral...

...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...Erin M Schuman, a former Caltech researcher, now Director at the Max Planck Institute for Brain Research...to all audiences. Right now, she is a science instructor at The Innovation Institute in Boston. When Eugenia...

...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...

...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...