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We narrowed to 971 results for: lars;

Showing: 651 - 660 of 971 results

  1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...packaging capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...cyanobacteria Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists...

  2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...CRISPR guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-...combinatorially modify the genome, screen for a particular phenotype, and quickly profile the resultant ...Sciences of the University of Hong Kong. He is particularly interested in developing and applying new technologies...

  3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...components. However, this sometimes requires intracellular injections into the cytoplasm or pronucleus ... albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades can also be used...

  4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...., 2020). Some Acr proteins are specific to a particular Cas protein while others can inhibit CRISPR enzymes...can increase the chance of off-target editing particularly after the majority of on-target editing has ...Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    .... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which... are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...point mutation or functional mutation in vivo: Similar to a conditional rescue experiment with a wildtype...sense (on) orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your...for monitoring Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802...

  7. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... parallel, this approach should be considered. Similar to the p24 assay, titering via measurement of viral...serial dilutions of a viral prep. This method is particularly useful when vectors carrying fluorescent markers...In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic resistance genes...

  8. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...moist and hopefully aerated with a thin layer of molecular biology-grade agarose containing physiological... advantage of this technique is the potentially large number of single clones obtained (on occasion we...Maryland Medical School in Baltimore. She is particularly interested in signaling peptide maturation in...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it has facilitated...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence or...

  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...yourself anymore. Personally I found this to be particularly challenging. As PhD students and postdocs we...there is not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions...manager for advice if you’re not sure how to face a particular situation. But keep in mind that a good manager...
Showing: 651 - 660 of 971 results