We narrowed to 1,073 results for: TOR;

...other researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene...cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each to make the ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

...yeast. These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5...from these yeast-optimized fluorophore tagging vectors for your single or multi-color imaging experiments...protease site)-6xGly linker-(epitope tag)-ADH1 terminator-(selection marker). Additionally, Dr. Formosa...

... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....includes 1049 community-validated reagents from 20 contributors, including 61 fluorescent probes, representing... showing the IBEX Knowledge-Base, with every contributor listed as an author.  The IBEX Knowledge-...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared by others...carrying out my studies, and getting them from collaborators was extremely helpful, but with hindsight I ...

...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...their lecture, giving them advice, [and helped] proctor exams.” These teaching experiences led Caitlin ...interview experience.” Although luck wasn’t the only factor in Caitlin landing her job. All of the hard work...

...bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested nearly 400 times, and... plasmids covering three different epigenetic effectors. The diversity of tools is especially exciting... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

...pre-clinical and human trial phases.   So how does CRISPR factor into this workflow? CRISPR both speeds up the process...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...breast cancer and identified several candidate inhibitors of gene X in a screen. You generate a gene X ...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...

... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...Addgene’s plasmid sharing services, as well as #happydepositors who stopped by to say hello. Needless to say...amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...sphaeroides!) at the ASM booth. As well as being the Director of the Great Lakes Bioenergy Research Center and...

...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...successful cloning. In these instances, the cloning vector typically has two separate resistance cassettes...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive...

...the table below for more details.   Common Vectors Copy Number+ ori Incompatibility Group Control ...Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility groups... ori's are referred to as phagemids.   Other factors that affect plasmid copy number  Although the...affect the copy number of a plasmid, other external factors contribute as well. These considerations are especially...