Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars;

Showing: 661 - 670 of 971 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...passive impact,” he says, noting that his team regularly shares troubleshooting tips and works with other...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...drug to the market usually takes over a billion dollars and at least a decade of work. The number of steps... Where publication is expected, the process is similar to academia. The only difference is that your paper...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  3. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: ...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it has facilitated...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence or...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid requests correlate...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...beautifully compelling photos and videos, while molecular, micro, and cell biology are often more suited...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls"   More...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...generating a frameshift mutation. The majority of cellular repair events will generate these types of mutations...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    ...hexon), as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong...Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected ...

  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... very likely find a useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental...
Showing: 661 - 670 of 971 results