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We narrowed to 1,073 results for: TOR;

Showing: 671 - 680 of 1073 results
  1. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...the extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant...antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate matrices, not...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...
  2. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ... first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...
  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...the table below for more details.   Common Vectors Copy Number+ ori Incompatibility Group Control ...Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility groups... ori's are referred to as phagemids.   Other factors that affect plasmid copy number  Although the...affect the copy number of a plasmid, other external factors contribute as well. These considerations are especially...
  4. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is...data prepared. All of these questions relate to factors that you should have already recorded as good reproducible...Chemogenetics) or light (Optogenetics) to activate receptors? If you’re in the process of designing your experiment...Addgene.org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...
  5. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...included a voltmeter on top of the battery box to monitor charge status and a disconnect switch between the... the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down.... can enable applications where reagents must be stored at elevated temperatures for long time periods ...
  6. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...CRISPRseek offers great utility and coupled with Bioconductor should be the first choice for anyone already...
  7. Synthetic Biology - Algal

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    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...
  8. Synthetic Biology - Bacterial

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...
  9. Synthetic Biology - Fungal

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...
Showing: 671 - 680 of 1073 results