... like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your...maximize cell density and plasmid yield as these factors are generally, but not perfectly, correlated. ...manufacturer. On Addgene plasmid pages, you may see depositor recommendations to grow a plasmid at 30℃ or room...

...population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within the genome...gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries ...library that is right for you There are several factors to consider when selecting a CRISPR library for...

...the sequencing results of the plasmids in our repository with known reference sequences, such as full ...full plasmid sequences provided by the laboratories that deposit their plasmids with us or other entries ...entries in NCBI’s numerous databases. As our repository has grown over the years (we now have over 60,000 plasmids...

...Protein (GFP), a protein from the jellyfish Aequorea Victoria. Since that discovery, the family of FPs just ... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...have a filter that will pass blue light to the detector/camera, then BFPs are of no use to you. When using...

... construct. This strategy can be used to build vectors with diverse functions, and thus, is used in many...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering...

...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...Further complicating the issue is the long and ugly history of reporting false essentialist links between biology...actively look for and consider bias as a confounding factor when interpreting findings, particularly when researching...

...colonies. The best way to do this is by using your vector with and without an insert to verify that the primers...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...PCR primers work in the first place: the backbone vector with and without an insert. These controls are ...

...number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his...

...applications? Fujii: We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral...joined Addgene's Advisory Board, as well as the Editorial Board of Scientific Reports and PLoS One. Additionally...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

... delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these...