Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
Type
Blog Post
..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs.
Hijacking tRNA processing...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...