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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 971 results for: lars;

Showing: 681 - 690 of 971 results

  1. Gibson Assembly Protocol

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson...effective method for the assembly of plasmids, and molecular biologists now use this method extensively. Why...be best when the DNA fragments are present in equimolar concentrations. The Gibson Assembly master mix...the important regions of your final plasmid, particularly the seams between the assembled parts. Tips ...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

  4. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  6. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... the gel in the first place. Carefully load a molecular weight ladder into the first lane of the gel. ...you are conducting certain procedures, such as molecular cloning, you will need to purify the DNA away ...you get better separation of bands? If you have similarly sized bands that are running too close together..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

  7. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...cytosine (C), guanine (G), thymine (T)] in an equal molar ratio. It also requires two unique single stranded...copy the DNA in between the oligo primers. This molecular biology technique creates several micrograms of...Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...frozen. DMSO helps with denaturation of the DNA, particularly for GC-rich templates. What does each ingredient...

  8. Molecular Biology Reference

    Type
    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... molecule is known as DNA or molecular cloning . The process of molecular cloning enabled scientists to...Basics Origins of Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids...Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes as carriers of phenotypic...These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA...chemistry enable isolation and purification of cellular components, such as DNA, but practically this ...

  9. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...blade Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant antibody...should be very little background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands...Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative intensity...

  10. AAV ddPCR Titration

    Type
    Protocol
    ... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes... lower or higher dilutions depending on their particular sample. For additional tips on AAV titering using... bucket 96-well freezer blocks (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp...10X Buffer 25 µL of 10% Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure Preparation Before...
Showing: 681 - 690 of 971 results