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We narrowed to 653 results for: EPO

Showing: 61 - 70 of 653 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The genes, from...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief...posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to ...growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens in human ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...murine CRISPR pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford...to use GMAP for your experiments, think about depositing your new plasmids with Addgene to help expand...

  2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...containing these mutations have been deposited at Addgene. Plasmids deposited from Alejandro Balazs’s lab includes...including a handful of plasmids that have been in the repository for many years, has now grown into a collection...lentivirus with the SARS-CoV-2 spike protein. They deposited the plasmids used for this study at Addgene which...can be completed in just 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase...  Drew Endy’s and Philippa Marrack’s labs also deposited plasmids for producing COVID-19 diagnostic enzymes...cathepsins play a role in SARS-CoV infection and deposited plasmids for the expression of cathepsin S, L,... of these proteins with human proteins. Their deposited plasmids expressed 26 of the 29 SARS-CoV-2 proteins...

  3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ... sequence or a sequence provided by the depositor. Depositing labs often provide insert sequences or annotated...by the depositing lab, we call these “quality control (QC) issues.” We then ask the depositing laboratory...take when performing quality control of newly deposited plasmids is to align the NGS result to a reference...laboratory to review the discrepancies. If the depositing laboratory confirms that these differences are expected...information about our sequencing results in the Depositor Comments section at the bottom of the plasmid .... In general, our QC scientists work with the depositing lab to develop a PCR strategy suitable for the...hope to make available as part of our OpenBio Repository sometime soon. Stay posted!     References ...

  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... versions of this reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27...The K9 reporter uses the HP1 chromodomain to recognise lysine 9 methylation, while the K27 reporter uses... methylation in living cells. Essentially this reporter construct consists of one substrate (the histone-derived...-K27 histone methylation reporter) positions on histone H3, which are known to be involved in repression...methylation of these sites was controlled, the reporter consistently showed significantly different FRET...states. Thus, this system is a reliable tool in reporting methylation status of these sites. This new technology...Alice Y. Ting. "Genetically encoded fluorescent reporters of histone methylation in living cells." Journal...

  5. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... added a new feature for depositing labs to its website, allowing depositors to download their plasmid...information can be of personal interest to the depositor, but it can also be valuable data to share in ...grant proposals or to demonstrate impact in annual reports. While the raw data is, of course, interesting ...is launched, it will show my data by default. Depositors can download their CSV file from Addgene by following...Dashboard After the upload, the name of the depositor and the date of downloading the CSV is shown (...and the source code is available in the Github repository (you can find the link in the upper right hand...dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim Goedhart is...

  6. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s most... to upload protocols when they deposit their plasmids to the repository. These protocols show up on the... and you decide to share them using Addgene’s repository, you can also share your associated protocols...“Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged...the benefits and how you can get started on our deposit page. Protocols.io Website: http://protocols.io...the software aims to be a life science protocol repository. The software can be used with the ZappyLabs ...Protocol Exchange (NPE) is an open online protocol repository hosted by the Nature Publishing Group. Like the...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...combination with that reporter, you can infer its presence through detecting the reporter's fluorescence. Another...labeling proteins is to fuse them with a fluorescent reporter, such as GFP or mCherry, through genetic modification...combination of antibody-fluorophores, fluorescent reporters, and/or dye stains used in your flow cytometry...mentioned previously, you can also use fluorescent reporters, such as GFP, and fluorophore-conjugated antibodies...compare the excitation/emission spectra of your reporter with the ones from your fluorophore labels and...encodes for eGFP, which serves as a transduction reporter. Note that eGFP is not fused to CD45, but is separately...for having a separately expressed transduction reporter is that not every transduced cell will necessarily...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...on creating a repository that made it easy to share plasmids, both for people depositing plasmids and ... materials in our repository. We started to increase accessibility to our repository by making it easier...to — so far, we’ve shipped materials from our repository to an amazing 111 countries (Figure 1)! But while...distributor allowed us to share materials and accept deposits from scientists in China. They were also able ...strides in increasing the accessibility of our repository, from physical materials to useful information...changes. It was only after creating a plasmid repository and shipping internationally, after all, that... addgene.org. Currently, we’re evaluating our depositor process to identify and reduce barriers for scientists...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...information from requestors and depositors, and identifying trends in our own repository data, giving us significant...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks ...order high quality viral vectors from the Addgene repository can save researchers significant time and removes...Cumulative AAV preps available in the Addgene repository each year from 2016–2023. B) AAV preps distributed...serotypes. In fact, the 879 viral vectors in our repository are produced from only 413 plasmids containing...different types of tools scientists were using/depositing. Our viral vectors data is less amenable to such...GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene...

  10. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    ...potential new deposits, and to meet with scientists throughout the world to encourage them to deposit. However...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...reach out to will end up depositing, we hope this campaign will expand the repository in ways that will be...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...
Showing: 61 - 70 of 653 results